Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit FOXL2 Polyclonal Antibody | anti-FOXL2 antibody
Phospho-FOXL2 (Ser238) Antibody
Gene Names
FOXL2; BPES; PFRK; POF3; BPES1; PINTO
Reactivity
Human, MousePredicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
FOXL2, Antibody; Phospho-FOXL2 (Ser238) Antibody; Blepharophimosis; Blepharophimosis epicanthus inversus and ptosis 1; Blepharophimosis epicanthus inversus and ptosis; BPES 1; BPES; BPES1; Epicanthus inversus and ptosis 1; Forkhead box L2; Forkhead box protein L2; Forkhead transcription factor FOXL2; FOX L2; FOXL 2; FOXL2; FOXL2_HUMAN; PFRK; PINTO; PITUITARY FORKHEAD FACTOR; POF 3; POF3; anti-FOXL2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-FOXL2 (Ser238) Antibody detects endogenous levels of FOXL2 only when phosphorylated at Ser238.
Tissue Specificity: In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
Tissue Specificity: In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-FOXL2 antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human FOXL2 around the phosphorylation site of Ser238.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Sumoylated with SUMO1; sumoylation is required for transcriptional repression activity.
Subunit Structure
Interacts with ESR1 (By similarity). Interacts with SMAD3 (By similarity). Interacts with DDX20. Interacts with UBE2I/UBC9.
Subcellular Location
Nucleus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using Phospho-FOXL2 (Ser238) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat , using Phospho-FOXL2 (Ser238) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Related Product Information for anti-FOXL2 antibody
Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,772 Da
NCBI Official Full Name
forkhead box protein L2
NCBI Official Synonym Full Names
forkhead box L2
NCBI Official Symbol
FOXL2
NCBI Official Synonym Symbols
BPES; PFRK; POF3; BPES1; PINTO
NCBI Protein Information
forkhead box protein L2; forkhead transcription factor FOXL2
UniProt Protein Name
Forkhead box protein L2
UniProt Gene Name
FOXL2
UniProt Entry Name
FOXL2_HUMAN
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Product Notes
The FOXL2 foxl2 (Catalog #AAA329465) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-FOXL2 (Ser238) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's FOXL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FOXL2 foxl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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