Rabbit FOXN1 Polyclonal Antibody | anti-FOXN1 antibody

FOXN1 Antibody (Center)

Cat. Num. AAA288090

• Gene Names: FOXN1; WHN; RONU; FKHL20
• Reactivity: Human; (Predicted: Mouse)
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FOXN1, Antibody; FOXN1 Antibody (Center); Forkhead box protein N1; Winged-helix transcription factor nude; FOXN1; RONU; WHN; anti-FOXN1 antibody

• Host: Rabbit
• Reactivity: Human; (Predicted: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB32003
• Specificity: This FOXN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-378 amino acids from the Central region of human FOXN1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 350-378
• Sequence Length: 648

• Applicable Applications for anti-FOXN1 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: Human
• Function: Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs). Essential for maintenance of mTECs population in the postnatal thymus. Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus. Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age. Essential for the vascularization of the thymus anlage. Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (By similarity). Plays a crucial role in the early prenatal stages of T-cell ontogeny (PubMed: 21507891).
• Cellular Location: Nucleus.
• Tissue Location: Expressed in thymus. Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. Amutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(FOXN1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(All lanes : Anti-FOXN1 Antibody (Center) at 1:2000 dilution Lane 1: Whole cell lysateLane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDaBlocking/Dilution buffer: 5% NFDM/TBST..)

Product Categories/Family for anti-FOXN1 antibody

Immunology

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Mandinova, A., et al. J. Clin. Invest. 119(10):3127-3137(2009)
Pignata, C., et al. Adv. Exp. Med. Biol. 665, 195-206 (2009) :
Weiner, L., et al. Cell 130(5):932-942(2007)
Nonaka, D., et al. Am. J. Surg. Pathol. 31(7):1038-1044(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 18201913
• NCBI GeneID: 8456
• NCBI Accession #: NP_003584.2
• NCBI GenBank Nucleotide #: NM_003593.2
• UniProt Accession #: O15353; O15352; B2R9Q7
• Molecular Weight: 68925
• NCBI Official Full Name: forkhead box protein N1
• NCBI Official Synonym Full Names: forkhead box N1
• NCBI Official Symbol: FOXN1
• NCBI Official Synonym Symbols: WHN; RONU; FKHL20
• NCBI Protein Information: forkhead box protein N1
• UniProt Protein Name: Forkhead box protein N1
• UniProt Gene Name: FOXN1
• UniProt Synonym Gene Names: RONU; WHN
• UniProt Entry Name: FOXN1_HUMAN