FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using FPGS Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human FPGS Polyclonal Antibody | anti-FPGS antibody
FPGS Antibody (Center)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FPGS, Antibody; FPGS Antibody (Center); Folylpolyglutamate synthase; mitochondrial; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; anti-FPGS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FPGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the Central region of human FPGS.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.33 (varies by lot)
Sequence Positions
304-330
Sequence Length
561
Applicable Applications for anti-FPGS antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using FPGS Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using FPGS Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of FPGS Antibody (Center) in K562, A2058 cell line lysates (35ug/lane). FPGS (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of FPGS Antibody (Center) in K562, A2058 cell line lysates (35ug/lane). FPGS (arrow) was detected using the purified Pab.)
Related Product Information for anti-FPGS antibody
FPGS is the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives.
Product Categories/Family for anti-FPGS antibody
References
Sharma,S., et.al., Pharmacogenet. Genomics 18 (12), 1041-1049 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64609
NCBI Official Full Name
folylpolyglutamate synthase, mitochondrial isoform b
NCBI Official Synonym Full Names
folylpolyglutamate synthase
NCBI Official Symbol
FPGS
NCBI Protein Information
folylpolyglutamate synthase, mitochondrial
UniProt Protein Name
Folylpolyglutamate synthase, mitochondrial
UniProt Gene Name
FPGS
UniProt Synonym Gene Names
FPGS; Tetrahydrofolate synthase
UniProt Entry Name
FOLC_HUMAN
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Product Notes
The FPGS fpgs (Catalog #AAA290154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 304-330. The FPGS Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FPGS can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FPGS fpgs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FPGS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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