IHC (Immunohiostchemistry)
(IHC image of AAA230584 diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit anti-Human, Rat Fructose-bisphosphate aldolase A Polyclonal Antibody | anti-ALDOA antibody
Rabbit anti-human Fructose-bisphosphate aldolase A polyclonal Antibody
Gene Names
ALDOA; ALDA; GSD12; HEL-S-87p
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
Fructose-bisphosphate aldolase A, Antibody; Rabbit anti-human Fructose-bisphosphate aldolase A polyclonal Antibody; Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Lung cancer antigen NY-LU-1) (Muscle-type aldolase), ALDOA, ALDA; anti-ALDOA antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Applicable Applications for anti-ALDOA antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human Fructose-bisphosphate aldolase A protein (2-364AA)
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Species
Human
Research Area
Signal Transduction
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-12061 / sc-12059 / sc-30082
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IHC (Immunohiostchemistry)
(IHC image of AAA230584 diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of AAA230584 diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, Rat kidney tissueAll lanes: ALDOA antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40, 46 kDaObserved band size: 46 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, Rat kidney tissueAll lanes: ALDOA antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40, 46 kDaObserved band size: 46 kDa)
Related Product Information for anti-ALDOA antibody
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein .Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.Belongs to the class I fructose-bisphosphate aldolase family.
Product Categories/Family for anti-ALDOA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fructose-bisphosphate aldolase A isoform 1
NCBI Official Synonym Full Names
aldolase, fructose-bisphosphate A
NCBI Official Symbol
ALDOA
NCBI Official Synonym Symbols
ALDA; GSD12; HEL-S-87p
NCBI Protein Information
fructose-bisphosphate aldolase A
UniProt Protein Name
Fructose-bisphosphate aldolase A
UniProt Gene Name
ALDOA
UniProt Synonym Gene Names
ALDA
UniProt Entry Name
ALDOA_HUMAN
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Product Notes
The ALDOA aldoa (Catalog #AAA230584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Fructose-bisphosphate aldolase A polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fructose-bisphosphate aldolase A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ALDOA aldoa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fructose-bisphosphate aldolase A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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