WB (Western Blot)
(Anti-GNAS rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:55 kDa)
Rabbit G protein alpha S/GNAS Polyclonal Antibody | anti-GNAS antibody
Anti-G protein alpha S/GNAS Antibody, Rabbit Polyclonal
Gene Names
GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45
Applications
Immunoprecipitation, Western Blot
Purity
ntigen Affinity Chromatography
Synonyms
G protein alpha S/GNAS, Antibody; Anti-G protein alpha S/GNAS Antibody, Rabbit Polyclonal; GNAS Antibody, Rabbit PAb, Antigen Affinity Purified; GNAS complex locus; Anti-AHO Antibody; Anti-C20orf45 Antibody; Anti-GNAS1 Antibody; Anti-GPSA Antibody; Anti-GSA Antibody; Anti-GSP Antibody; Anti-NESP Antibody; Anti-PHP1A Antibody; Anti-PHP1B Antibody; Anti-PHP1C Antibody; Anti-POH Antibody; Anti-SgVI Antibody; anti-GNAS antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GNAS
Purity/Purification
ntigen Affinity Chromatography
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-GNAS antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human GNAS
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human GNAS, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles. Sodium Azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
WB (Western Blot)
(Anti-GNAS rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:55 kDa)
WB (Western Blot)
(Anti-GNAS rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:55 kDa)
IP (Immunoprecipitation)
(GNAS was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-GNAS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GNAS rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)
IP (Immunoprecipitation)
(GNAS was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-GNAS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GNAS rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)
Related Product Information for anti-GNAS antibody
GNAS (GNAS Complex Locus) is a Protein Coding gene. GNAS is a complex imprinted gene encoding the alpha-subunit of the stimulatory heterotrimeric G protein (Gs alpha), which belongs to the G-alpha family. Inactivating mutations in Gsalpha-coding GNAS exons are responsible for Albright's hereditary osteodystrophy (AHO), which refers to a constellation of physical and developmental disorders including obesity, short stature, brachydactyly, cognitive impairment, and heterotopic ossification. GNAS mediates receptor-stimulated cAMP signaling, which integrates diverse environmental cues with intracellular responses. GNAS mutations have been described in mucinous and non-mucinous epithelial neoplasms of the appendix, pancreas, and colon, with hotspot GNAS mutations found in up to two-thirds of pancreatic intraductal papillary mucinous neoplasms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,948 Da
NCBI Official Full Name
protein ALEX Alex
NCBI Official Synonym Full Names
GNAS complex locus
NCBI Official Symbol
GNAS
NCBI Official Synonym Symbols
AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45
NCBI Protein Information
protein ALEX; protein GNAS; protein SCG6 (secretogranin VI)
UniProt Protein Name
Protein ALEX
UniProt Gene Name
GNAS
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Product Notes
The GNAS gnas (Catalog #AAA254358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's G protein alpha S/GNAS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GNAS gnas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G protein alpha S/GNAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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