IF (Immunofluorescence)
(AAA328992 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328992 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit GABA A Receptor beta 3 Polyclonal Antibody | anti-GABRB3 antibody
GABA A Receptor beta 3 Antibody
Gene Names
GABRB3; ECA5; EIEE43
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
GABA A Receptor beta 3, Antibody; GABA A Receptor beta 3 Antibody; ECA5; GABA alpha receptor beta-2 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta 3 subunit; GABAA receptor subunit beta 3; GABR B3; Gabrb3; Gamma aminobutyric acid (GABA) A receptor beta 3; Gamma aminobutyric acid receptor subunit beta 3; Gamma-aminobutyric acid receptor subunit beta-3; GBRB3_HUMAN; MGC9051; anti-GABRB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Chicken(100%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Chicken(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
GABA A Receptor beta 3 Antibody detects endogenous levels of total GABA A Receptor beta 3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
473
Applicable Applications for anti-GABRB3 antibody
ELISA
Immunogen
A synthesized peptide derived from human GABA A Receptor beta 3, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328992 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328992 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328992 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328992 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from Rat brain, using GABA A Receptor beta 3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Rat brain, using GABA A Receptor beta 3 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-GABRB3 antibody
Function: Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:18514161, PubMed:22303015, PubMed:26950270, PubMed:22243422, PubMed:24909990). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:25489750). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (By similarity). The alpha1/beta3/gamma2 receptor exhibits synaptogenic activity (PubMed:25489750). The alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (By similarity). Functions also as histamine receptor and mediates cellular responses to histamine (PubMed:18281286). Plays an important role in somatosensation and in the production of antinociception (By similarity).
Subcellular Location: Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane.
Subunit Structure: Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains (PubMed:22243422, PubMed:18281286, PubMed:18514161, PubMed:24909990). Can form functional homopentamers (in vitro) (PubMed:22303015). Interacts with UBQLN1 (By similarity). May interact with KIF21B (By similarity). Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (By similarity). Interacts with LHFPL4 (By similarity).
Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.
Subcellular Location: Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane.
Subunit Structure: Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains (PubMed:22243422, PubMed:18281286, PubMed:18514161, PubMed:24909990). Can form functional homopentamers (in vitro) (PubMed:22303015). Interacts with UBQLN1 (By similarity). May interact with KIF21B (By similarity). Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (By similarity). Interacts with LHFPL4 (By similarity).
Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)54kD.
Predicted Molecular Weight: (Calculated)54kDa.
Predicted Molecular Weight: (Calculated)54kDa.
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit beta-3 isoform 1
NCBI Official Synonym Full Names
gamma-aminobutyric acid type A receptor subunit beta3
NCBI Official Symbol
GABRB3
NCBI Official Synonym Symbols
ECA5; EIEE43
NCBI Protein Information
gamma-aminobutyric acid receptor subunit beta-3
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit beta-3
UniProt Gene Name
GABRB3
UniProt Entry Name
GBRB3_HUMAN
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Product Notes
The GABRB3 gabrb3 (Catalog #AAA328992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GABA A Receptor beta 3 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Chicken(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's GABA A Receptor beta 3 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the GABRB3 gabrb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABA A Receptor beta 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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