Goat anti-Human, Mouse GAD67 Polyclonal Antibody | anti-GAD67 antibody

Goat Polyclonal to Human GAD67

Cat. Num. AAA51000

• Gene Names: GAD1; GAD; SCP; CPSQ1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GAD67, Antibody; Goat Polyclonal to Human GAD67; Anti-GAD67 Antibody (Internal) IHC-plus; GAD1; CPSQ1; GAD-67; Glutamate decarboxylase 1; GAD67; GAD; SCP; Human GAD67; anti-GAD67 antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Human GAD11 GAD67. This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Positions: 526-537
• Sequence Length: 594

• Applicable Applications for anti-GAD67 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: GAD1 1 GAD67 antibody was raised against Human
• Immunogen: GAD1 1 GAD67 antibody was raised against peptide with sequence CPDSPQRREKLHK, from the internal region of the protein sequence according to NP _000808.2.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect tohow this antibody will behave in your system. Researchers using this antibody should conduct optimization studies to achieve themost optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes noguarantee of the results:Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

WB (Western Blot)

(Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry)

(Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Related Product Information for anti-GAD67 antibody

Immunohistochemistry: MBS242634 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for 242634 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1 :8000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain Iysates (calculated MW of 66.9kD according to Human NP 000808.2 and 66.6kD according to Mouse NP _032103.2). Recommended concentration: 0.3-1 ug/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 58331246
• NCBI GeneID: 2571
• NCBI Accession #: NP_000808.2
• NCBI GenBank Nucleotide #: NM_000817.2
• UniProt Accession #: Q99259; Q49AK1; Q53TQ7; Q9BU91; Q9UHH4
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: glutamate decarboxylase 1 isoform GAD67
• NCBI Official Synonym Full Names: glutamate decarboxylase 1 (brain, 67kDa)
• NCBI Official Symbol: GAD1
• NCBI Official Synonym Symbols: GAD; SCP; CPSQ1
• NCBI Protein Information: glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform
• UniProt Protein Name: Glutamate decarboxylase 1
• UniProt Gene Name: GAD1
• UniProt Synonym Gene Names: GAD; GAD67; GAD-67
• UniProt Entry Name: DCE1_HUMAN