FCM/FACS (Flow Cytometry)
(GADD45A Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit GADD45A Polyclonal Antibody | anti-GADD45A antibody
GADD45A Antibody (N-term)
Gene Names
GADD45A; DDIT1; GADD45
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GADD45A, Antibody; GADD45A Antibody (N-term); Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1; GADD45A; DDIT1; GADD45; anti-GADD45A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GADD45A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1-30
Sequence Length
131
Applicable Applications for anti-GADD45A antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GADD45A Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GADD45A Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.)
WB (Western Blot)
(Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.)
WB (Western Blot)
(Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.)
Related Product Information for anti-GADD45A antibody
GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
References
Li,L.S., J. Biol. Chem. 283 (31), 21394-21403 (2008) Al-Romaih,K., Neoplasia 10 (5), 471-480 (2008) Zhu,Q.S., Cancer Res. 68 (8), 2895-2903 (2008) Kearsey,J.M., Oncogene 11 (9), 1675-1683 (1995)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18336
NCBI Official Full Name
growth arrest and DNA damage-inducible protein GADD45 alpha isoform 2
NCBI Official Synonym Full Names
growth arrest and DNA-damage-inducible, alpha
NCBI Official Symbol
GADD45A
NCBI Official Synonym Symbols
DDIT1; GADD45
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Gene Name
GADD45A
UniProt Synonym Gene Names
DDIT1; GADD45; DDIT-1
UniProt Entry Name
GA45A_HUMAN
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Product Notes
The GADD45A gadd45a (Catalog #AAA285590) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The GADD45A Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's GADD45A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GADD45A gadd45a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GADD45A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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