FCM/FACS (Flow Cytometry)
(GAGE7 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human GAGE7 Polyclonal Antibody | anti-GAGE7 antibody
GAGE7 Antibody (C-term)
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GAGE7, Antibody; GAGE7 Antibody (C-term); G antigen 7; GAGE-7; AL4; Cancer/testis antigen 47; CT47; GAGE-12I; GAGE-7B; GAGE-8; GAGE7; GAGE12I; GAGE7B; anti-GAGE7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This GAGE7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the C-terminal region of human GAGE7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
87-116
Sequence Length
117
Applicable Applications for anti-GAGE7 antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Tissue Location
Expressed in some prostate cancer tissues but not in normal prostate tissue
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GAGE7 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GAGE7 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(GAGE7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAGE7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GAGE7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAGE7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of GAGE7 Antibody (C-term) in K562 cell line lysates (35ug/lane). GAGE7 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of GAGE7 Antibody (C-term) in K562 cell line lysates (35ug/lane). GAGE7 (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-GAGE7 antibody
References
Gjerstorff, M.F., et al. Tissue Antigens 71(3):187-192(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 12978
NCBI Official Full Name
G antigen 12F
NCBI Official Synonym Full Names
G antigen 12F
NCBI Official Symbol
GAGE12F
NCBI Protein Information
G antigen 12F
UniProt Protein Name
G antigen 7
UniProt Gene Name
GAGE7
UniProt Synonym Gene Names
GAGE12I; GAGE7B; GAGE-7; CT4.7
UniProt Entry Name
GAGE7_HUMAN
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Product Notes
The GAGE7 gage7 (Catalog #AAA289047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 87-116. The GAGE7 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAGE7 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GAGE7 gage7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAGE7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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