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IF (Immunofluorescence)
(Immunofluorescence staining of GAK in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GAK polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human Gak Polyclonal Antibody | anti-Gak antibody
Anti-Gak Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Gak, Antibody; Anti-Gak Antibody, Rabbit Polyclonal; Gak Antibody, Rabbit PAb, Antigen Affinity Purified; anti-Gak antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Gak
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-Gak antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human Gak fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human Gak fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of GAK in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GAK polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of GAK in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GAK polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohistochemisry)
(Immunochemical staining of human GAK in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human GAK in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human GAK in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human GAK in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-GAK rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:143 kDaObserved band size: kDa)
WB (Western Blot)
(Anti-GAK rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:143 kDaObserved band size: kDa)
Related Product Information for anti-Gak antibody
Cyclin G-associated kinase (GAK), also known as auxilin 2, is a member of the Numb-associated kinase (NAK) family. Other members include adaptor-associated kinase 1 (AAK1), BMP2-inducible kinase (BIKE/BMP2), and myristoylated and palmitoylated serine/threonine kinase 1 (MPSK1). GAK is ubiquitously expressed in various tissues and is primarily known for its role in clathrin uncoating. At its N-terminus, GAK has a highly conserved serine/threonine kinase domain followed by a lipid-binding PTEN-like domain, a clathrin-binding domain, and a J-domain, the latter two domains being sufficient for Hsc70 and GAK-mediated uncoating of clathrin-coated vesicles. As a serine/threonine kinase, GAK exerts part of its functional effects by targeting downstream substrates. The only currently known substrates of GAK are theu2-andu1-subunits of the adaptor proteins AP-2 and AP-1, respectively, and protein phosphatase 2A.
References
Lin AW, et al. (2018) Chemical genetic identification of gak substrates reveals its role in regulating na(+)/k(+)-atpase. Life Sci Alliance 1 (6): e201800118.
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Product Notes
The Gak (Catalog #AAA256552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Gak Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Gak can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Gak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gak, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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