FCM/FACS (Flow Cytometry)
(GALNT5 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human GALNT5 Polyclonal Antibody | anti-GALNT5 antibody
GALNT5 Antibody (N-term)
Gene Names
GALNT5; GALNACT5; GALNAC-T5
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GALNT5, Antibody; GALNT5 Antibody (N-term); Polypeptide N-acetylgalactosaminyltransferase 5; Polypeptide GalNAc transferase 5; GalNAc-T5; pp-GaNTase 5; Protein-UDP acetylgalactosaminyltransferase 5; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5; GALNT5; anti-GALNT5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GALNT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human GALNT5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
24-53
Sequence Length
940
Applicable Applications for anti-GALNT5 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GALNT5 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GALNT5 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(GALNT5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human normal lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GALNT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GALNT5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human normal lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GALNT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from A431, K562 cell line (from left to right), using GALNT5 Antibody (N-term). AAA287654 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, K562 cell line (from left to right), using GALNT5 Antibody (N-term). AAA287654 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-GALNT5 antibody
GALNT5 can catalyze the initial reaction in O-linked oligosaccharide biosynthesis,the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT5 has activity toward EA2 peptide substrate, but it has a weak activity toward Muc2 or Muc1b substrates.
Product Categories/Family for anti-GALNT5 antibody
References
Simmons, A.D., et al. Hum. Mol. Genet. 8(12):2155-2164(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106266
NCBI Official Full Name
polypeptide N-acetylgalactosaminyltransferase 5
NCBI Official Synonym Full Names
polypeptide N-acetylgalactosaminyltransferase 5
NCBI Official Symbol
GALNT5
NCBI Official Synonym Symbols
GALNACT5; GALNAC-T5
NCBI Protein Information
polypeptide N-acetylgalactosaminyltransferase 5
UniProt Protein Name
Polypeptide N-acetylgalactosaminyltransferase 5
UniProt Gene Name
GALNT5
UniProt Synonym Gene Names
GalNAc-T5; pp-GaNTase 5
UniProt Entry Name
GALT5_HUMAN
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Product Notes
The GALNT5 galnt5 (Catalog #AAA287654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 24-53. The GALNT5 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the GALNT5 galnt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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