<
WB (Western Blot)
(Western blot analysis of HEK293 cell lysate, using GARS Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit GARS Polyclonal Antibody | anti-GARS antibody
GARS Antibody
Gene Names
GARS; HMN5; CMT2D; DSMAV; GlyRS; SMAD1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GARS, Antibody; GARS Antibody; AP 4 A synthetase; Charcot Marie Tooth neuropathy 2D; Charcot Marie Tooth neuropathy neuronal type D; CMT2D; Diadenosine tetraphosphate synthetase; DSMAV; EC 6.1.1.14; Glycine tRNA ligase; Glycyl tRNA synthetase; GlyRS; HMN5; SMAD1; anti-GARS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GARS antibody detects endogenous levels of total GARS
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
685
Applicable Applications for anti-GARS antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human GARS
Subcellular Location
Cytoplasm. Mitochondrion.
Tissue Specificity
Widely expressed, including in brain and spinal cord.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of HEK293 cell lysate, using GARS Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of HEK293 cell lysate, using GARS Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from rat spleen, using GARS Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat spleen, using GARS Antibody.)
IHC (Immunohiostchemistry)
(AAA327041 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327041 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, rat liver, mouse liver, mouse muscle, using GARS Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, rat liver, mouse liver, mouse muscle, using GARS Antibody.)
Related Product Information for anti-GARS antibody
Description: This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
Function: Catalyzes the ligation of glycine to the 3'-end of its cognate tRNA. Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Subunit Structure: Homodimer.
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family.
Function: Catalyzes the ligation of glycine to the 3'-end of its cognate tRNA. Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Subunit Structure: Homodimer.
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 75 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
glycine--tRNA ligase isoform 2
NCBI Official Synonym Full Names
glycyl-tRNA synthetase
NCBI Official Symbol
GARS
NCBI Official Synonym Symbols
HMN5; CMT2D; DSMAV; GlyRS; SMAD1
NCBI Protein Information
glycine--tRNA ligase
UniProt Protein Name
Glycine--tRNA ligase
UniProt Gene Name
GARS
UniProt Synonym Gene Names
AP-4-A synthetase; GlyRS
Similar Products
Product Notes
The GARS gars (Catalog #AAA327041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GARS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GARS can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GARS gars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.