WB (Western Blot)
(Detection of human GATA4 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, SK-N-MC, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GATA4 antibody (AAA212194 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human GATA4 Polyclonal Antibody | anti-GATA4 antibody
Rabbit anti-GATA4 Antibody, Affinity Purified
Gene Names
GATA4; ASD2; VSD1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GATA4, Antibody; Rabbit anti-GATA4 Antibody, Affinity Purified; ASD2; GATA-binding factor 4; TACHD; TOF; transcription factor GATA-4; VSD1; Transcription factor GATA-4; GATA binding protein 4; anti-GATA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GATA4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 392 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GATA4 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, SK-N-MC, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GATA4 antibody (AAA212194 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human GATA4 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, SK-N-MC, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GATA4 antibody (AAA212194 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GATA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GATA4 antibody (AAA212194 lot 2) used for IP at 6 ug per reaction. GATA4 was also immunoprecipitated by a previous lot of this antibody (AAA212194 lot 1) and a second antibody against a different epitope of GATA4 (BL12492). For blotting immunoprecipitated GATA4, AAA212194 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human GATA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GATA4 antibody (AAA212194 lot 2) used for IP at 6 ug per reaction. GATA4 was also immunoprecipitated by a previous lot of this antibody (AAA212194 lot 1) and a second antibody against a different epitope of GATA4 (BL12492). For blotting immunoprecipitated GATA4, AAA212194 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human GATA4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-GATA4 (Cat. No. AAA212194 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human GATA4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-GATA4 (Cat. No. AAA212194 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-GATA4 antibody
GATA binding protein 4 (GATA4) transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3' and promotes cardiac myocyte enlargement.
Product Categories/Family for anti-GATA4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,565 Da
NCBI Official Full Name
transcription factor GATA-4
NCBI Official Synonym Full Names
GATA binding protein 4
NCBI Official Symbol
GATA4
NCBI Official Synonym Symbols
ASD2; VSD1
NCBI Protein Information
transcription factor GATA-4; GATA-binding factor 4
UniProt Protein Name
Transcription factor GATA-4
UniProt Gene Name
GATA4
UniProt Entry Name
GATA4_HUMAN
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Product Notes
The GATA4 gata4 (Catalog #AAA212194) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GATA4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GATA4 gata4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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