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Application Data
(AtT-20 transfected with GFP-Rab27a and immunostained with GFP Ab)
Goat GFP Polyclonal Antibody | anti-GFP antibody
GFP Polyclonal Antibody
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Synonyms
GFP, Antibody; GFP Polyclonal Antibody; anti-GFP; anti-GFP antibody
Host
Goat
Clonality
Polyclonal
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recogize RFP and mCherry fluorescent proteins.
Form/Format
Supplied as (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Sequence Length
238
Applicable Applications for anti-GFP antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence)
Immunogen
Purified recombinant peptide produced in E. coli.
Preparation and Storage
Store at -20°C for long term storage. Store at 2-8°C for short term. Avoid freeze/thaw cycles.
Application Data
(AtT-20 transfected with GFP-Rab27a and immunostained with GFP Ab)
Application Data
(AtT-20 transfected with GFP-Rab27a and immunostained with GFP Ab)
IHC (Immunohistochemisry)
(AtT-20 transfected with GFP-Rab27a and immunostained with GFP Ab)
IHC (Immunohistochemisry)
(AtT-20 transfected with GFP-Rab27a and immunostained with GFP Ab)
IHC (Immunohiostchemistry)
(Primary fibroblasts transduced with Ad GFP-Rabla and immunostained with GFP Ab)
IHC (Immunohiostchemistry)
(Primary fibroblasts transduced with Ad GFP-Rabla and immunostained with GFP Ab)
WB (Western Blot)
(Anti-GFP at 1/1000 dilution; HEK293 trasnfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution)
WB (Western Blot)
(Anti-GFP at 1/1000 dilution; HEK293 trasnfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution)
Related Product Information for anti-GFP antibody
Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
References
1. Pais SV, Milho C, Almeida F, Mota LJ. PLoS One. 2013;8(2):e56292. doi: 10.1371/journal.pone.0056292. Epub 2013 Feb 19.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
GFP
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Product Notes
The GFP (Catalog #AAA63068) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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