IP (Immunoprecipitation)
(GFP was immunoprecipitated using:; Lane A:0.5 mg pCMV3-untagged Negative Control Vector; Lane B:0.5 mg pCMV3-C-GFPSpark Vector; Lane C:0.5 mg pCMV3-N-GFPSpark Vector; Lane D:0.5 mg pLV-untagged Lentivirus Control Plasmid; Lane E:0.5 mg pLV-N-GFPSpark Lentivirus Control Plasmid; 4 uL anti- GFP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- GFP rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.)
Rabbit GFP Polyclonal Antibody | anti-GFP antibody
GFP Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Aequorea victoria
Applications
Immunoprecipitation, ELISA, Western Blot
Synonyms
GFP, Antibody; GFP Antibody, Rabbit PAb, Antigen Affinity Purified; anti-GFP antibody
Host
Rabbit
Reactivity
Aequorea victoria
Clonality
Polyclonal
Specificity
Recognize GFP, EGFP, GFPSpark
Applicable Applications for anti-GFP antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Preparation
Produced in rabbits immunized with purified, recombinant Aequorea victoria GFP;. GFP specific IgG was purified by Aequorea victoria GFP affinity chromatography.
Cross-reactivity in ELISA
Has cross-reactivity in ELISA and WB with
Aequorea victoria GFP Protein EGFP/Enhanced Green Fluorescent ProteinAequorea victoria Green fluorescent protein /enhanced GFP GFPSparkTM
Aequorea victoria GFP Protein EGFP/Enhanced Green Fluorescent ProteinAequorea victoria Green fluorescent protein /enhanced GFP GFPSparkTM
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(GFP was immunoprecipitated using:; Lane A:0.5 mg pCMV3-untagged Negative Control Vector; Lane B:0.5 mg pCMV3-C-GFPSpark Vector; Lane C:0.5 mg pCMV3-N-GFPSpark Vector; Lane D:0.5 mg pLV-untagged Lentivirus Control Plasmid; Lane E:0.5 mg pLV-N-GFPSpark Lentivirus Control Plasmid; 4 uL anti- GFP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- GFP rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.)
IP (Immunoprecipitation)
(GFP was immunoprecipitated using:; Lane A:0.5 mg pCMV3-untagged Negative Control Vector; Lane B:0.5 mg pCMV3-C-GFPSpark Vector; Lane C:0.5 mg pCMV3-N-GFPSpark Vector; Lane D:0.5 mg pLV-untagged Lentivirus Control Plasmid; Lane E:0.5 mg pLV-N-GFPSpark Lentivirus Control Plasmid; 4 uL anti- GFP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- GFP rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.)
Application Data
(Anti-GFP rabbit monoclonal antibody at 1:1000 dilution.; Sample: Reocombinant Protein 10 ng; Lane 1: Aequorea victoria GFP Protein (His Tag); Lane 2: EGFP/Enhanced Green Fluorescent Protein (His Tag); Lane 3: Aequorea victoria Green fluorescent protein /enhanced GFP GFPSparkTM (His Tag); Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.)
Application Data
(Anti-GFP rabbit monoclonal antibody at 1:1000 dilution.; Sample: Reocombinant Protein 10 ng; Lane 1: Aequorea victoria GFP Protein (His Tag); Lane 2: EGFP/Enhanced Green Fluorescent Protein (His Tag); Lane 3: Aequorea victoria Green fluorescent protein /enhanced GFP GFPSparkTM (His Tag); Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.)
Related Product Information for anti-GFP antibody
Green fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins.
NCBI and Uniprot Product Information
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Product Notes
The GFP (Catalog #AAA258638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GFP Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Aequorea victoria and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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