FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-GGA2 antibody (AAA127210).Overlay histogram showing HepG2 cells stained with AAA127210 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA2 Antibody (AAA127210, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human GGA2 Polyclonal Antibody | anti-GGA2 antibody
Anti-GGA2 Antibody Picoband
Gene Names
GGA2; VEAR
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
GGA2, Antibody; Anti-GGA2 Antibody Picoband; anti-GGA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-GGA2 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human GGA2 recombinant protein (Position: L26-A608). Human GGA2 shares 85.4% amino acid (aa) sequence identity with mouse GGA2.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-GGA2 antibody (AAA127210).Overlay histogram showing HepG2 cells stained with AAA127210 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA2 Antibody (AAA127210, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-GGA2 antibody (AAA127210).Overlay histogram showing HepG2 cells stained with AAA127210 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA2 Antibody (AAA127210, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of GGA2 using anti-GGA2 antibody (AAA127210).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (#AAA127210) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of GGA2 using anti-GGA2 antibody (AAA127210).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (#AAA127210) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.)
Related Product Information for anti-GGA2 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-GGA2 antibody
References
1. Dell'Angelica, E. C., Puertollano, R., Mullins, C., Aguilar, R. C., Vargas, J. D., Hartnell, L. M., Bonifacino, J. S. GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. J. Cell Biol. 149: 81-93, 2000. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/23/2017.3. Hirst, J., Lui, W. W. Y., Bright, N. A., Totty, N., Seaman, M. N. J., Robinson, M. S. A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome. J. Cell Biol. 149: 67-69, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
613
NCBI Official Full Name
ADP-ribosylation factor-binding protein GGA2
NCBI Official Synonym Full Names
golgi-associated, gamma adaptin ear containing, ARF binding protein 2
NCBI Official Symbol
GGA2
NCBI Official Synonym Symbols
VEAR
NCBI Protein Information
ADP-ribosylation factor-binding protein GGA2; gamma-adaptin-related protein 2; VHS domain and ear domain of gamma-adaptin; VHS domain and ear domain-containing protein; golgi-localized, gamma ear-containing, ARF-binding protein 2; golgi associated, gamma
UniProt Protein Name
ADP-ribosylation factor-binding protein GGA2
UniProt Gene Name
GGA2
UniProt Synonym Gene Names
KIAA1080; Vear
UniProt Entry Name
GGA2_HUMAN
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Product Notes
The GGA2 gga2 (Catalog #AAA127210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GGA2 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GGA2 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the GGA2 gga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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