IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
Rabbit anti-Human, Mouse GLI1 Polyclonal Antibody | anti-GLI1 antibody
GLI1 Antibody
Gene Names
GLI1; GLI
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
GLI1, Antibody; GLI1 Antibody; GLI; anti-GLI1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total GLI1 protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.5mg/ml (varies by lot)
Sequence Length
978
Applicable Applications for anti-GLI1 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Synthetic peptide of human GLI1
Immunogen Type
Peptide
Calculated MW
118kd
Preparation and Storage
Store at -20°C
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
SDS-PAGE
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane 1-4: NIH/3T3£¬Raji£¬PC-3 and Hepg2 cell, Primary antibody:GLI1 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
SDS-PAGE
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane 1-4: NIH/3T3£¬Raji£¬PC-3 and Hepg2 cell, Primary antibody:GLI1 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
Related Product Information for anti-GLI1 antibody
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-GLI1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein GLI1 isoform 2
NCBI Official Synonym Full Names
GLI family zinc finger 1
NCBI Official Symbol
GLI1
NCBI Official Synonym Symbols
GLI
NCBI Protein Information
zinc finger protein GLI1
UniProt Protein Name
Zinc finger protein GLI1
UniProt Gene Name
GLI1
UniProt Synonym Gene Names
GLI
UniProt Entry Name
GLI1_HUMAN
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Product Notes
The GLI1 gli1 (Catalog #AAA309391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLI1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLI1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the GLI1 gli1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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