WB (Western Blot)
(Western blot analysis of lysates from 293, Jurkat cell line (from left to right), using GLI2 Antibody (C-term). AAA285931 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit anti-Human GLI2 Polyclonal Antibody | anti-GLI2 antibody
GLI2 Antibody (C-term)
Gene Names
GLI2; CJS; HPE9; PHS2; THP1; THP2
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GLI2, Antibody; GLI2 Antibody (C-term); Zinc finger protein GLI2; GLI family zinc finger protein 2 {ECO:0000312|HGNC:HGNC:4318}; Tax helper protein; GLI2 HGNC:4318); anti-GLI2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GLI2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1287-1321 amino acids from the C-terminal region of human GLI2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1586
Applicable Applications for anti-GLI2 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from 293, Jurkat cell line (from left to right), using GLI2 Antibody (C-term). AAA285931 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from 293, Jurkat cell line (from left to right), using GLI2 Antibody (C-term). AAA285931 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using GLI2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA285931 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using GLI2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA285931 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with GLI2 Antibody (C-term). AAA285931 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with GLI2 Antibody (C-term). AAA285931 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-GLI2 antibody
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Is involved in the smoothened (SHH) signaling pathway.
Product Categories/Family for anti-GLI2 antibody
References
Tanimura A.,et al.J. Virol. 72:3958-3964(1998).
Roessler E.,et al.Hum. Mol. Genet. 14:2181-2188(2005).
Hillier L.W.,et al.Nature 434:724-731(2005).
Tanimura A.,et al.J. Virol. 67:5375-5382(1993).
Ruppert J.M.,et al.Mol. Cell. Biol. 8:3104-3113(1988).
Roessler E.,et al.Hum. Mol. Genet. 14:2181-2188(2005).
Hillier L.W.,et al.Nature 434:724-731(2005).
Tanimura A.,et al.J. Virol. 67:5375-5382(1993).
Ruppert J.M.,et al.Mol. Cell. Biol. 8:3104-3113(1988).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
167783
NCBI Official Full Name
zinc finger protein GLI2
NCBI Official Synonym Full Names
GLI family zinc finger 2
NCBI Official Symbol
GLI2
NCBI Official Synonym Symbols
CJS; HPE9; PHS2; THP1; THP2
NCBI Protein Information
zinc finger protein GLI2
UniProt Protein Name
Zinc finger protein GLI2
UniProt Gene Name
GLI2
UniProt Entry Name
GLI2_HUMAN
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Product Notes
The GLI2 gli2 (Catalog #AAA285931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLI2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GLI2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GLI2 gli2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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