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IHC (Immunohistochemistry)
(GLS2 antibody(C-term E513) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit GLS2 Polyclonal Antibody | anti-GLS2 antibody
GLS2 Antibody (C-term E513)
Gene Names
GLS2; GA; GLS; LGA; hLGA
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GLS2, Antibody; GLS2 Antibody (C-term E513); Glutaminase liver isoform; mitochondrial; GLS; L-glutaminase; L-glutamine amidohydrolase; GLS2; GA; anti-GLS2 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GLS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 498-524 amino acids from the C-terminal region of human GLS2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
498-524
Sequence Length
337
Applicable Applications for anti-GLS2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(GLS2 antibody(C-term E513) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(GLS2 antibody(C-term E513) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(GLS2 Antibody (C-term E513) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GLS2 antibody detected the GLS2 protein (arrow).)
WB (Western Blot)
(GLS2 Antibody (C-term E513) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GLS2 antibody detected the GLS2 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysate from human brain tissue lysate, using GLS2 Antibody (C-term E513). AAA285896 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from human brain tissue lysate, using GLS2 Antibody (C-term E513). AAA285896 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513). AAA285896 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513). AAA285896 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-GLS2 antibody
The protein encoded by this gene is a mitochondrial
phosphate-activated glutaminase that catalyzes the hydrolysis of
glutamine to stoichiometric amounts of glutamate and ammonia. This
protein is functionally similar to the kidney glutaminase but is a
little smaller in size. Originally thought to be liver-specific,
this protein has been found in other tissues as well. At least one
transcribed pseudogene has been found for this gene. [provided by
RefSeq].
phosphate-activated glutaminase that catalyzes the hydrolysis of
glutamine to stoichiometric amounts of glutamate and ammonia. This
protein is functionally similar to the kidney glutaminase but is a
little smaller in size. Originally thought to be liver-specific,
this protein has been found in other tissues as well. At least one
transcribed pseudogene has been found for this gene. [provided by
RefSeq].
Product Categories/Family for anti-GLS2 antibody
References
Hu, W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7455-7460(2010)
Suzuki, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7461-7466(2010)
Szeliga, M., et al. Glia 57(9):1014-1023(2009)
Tian, C., et al. J. Neurochem. 105(3):994-1005(2008)
Maeshima, H., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 31(7):1410-1418(2007)
Suzuki, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7461-7466(2010)
Szeliga, M., et al. Glia 57(9):1014-1023(2009)
Tian, C., et al. J. Neurochem. 105(3):994-1005(2008)
Maeshima, H., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 31(7):1410-1418(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66323
NCBI Official Full Name
glutaminase liver isoform, mitochondrial isoform 4
NCBI Official Synonym Full Names
glutaminase 2 (liver, mitochondrial)
NCBI Official Symbol
GLS2
NCBI Official Synonym Symbols
GA; GLS; LGA; hLGA
NCBI Protein Information
glutaminase liver isoform, mitochondrial
UniProt Protein Name
Glutaminase liver isoform, mitochondrial
UniProt Gene Name
GLS2
UniProt Synonym Gene Names
GA; GLS
UniProt Entry Name
GLSL_HUMAN
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Product Notes
The GLS2 gls2 (Catalog #AAA285896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 498-524. The GLS2 Antibody (C-term E513) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GLS2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GLS2 gls2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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