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product-image-AAA44918_IHC10.jpg IHC (Immunohistochemistry) (Figure 4. IHC analysis of GLUT5 using anti- GLUT5 antibodyGLUT5 was detected in paraffin-embedded section of mouse ggj3249 tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- GLUT5 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Rabbit anti-Mouse, Rat Glucose Transporter 5 GLUT5 Polyclonal Antibody | anti-SLC2A5 antibody

Polyclonal Anti-SLC2A5 Antibody

Gene Names
Slc2a5; Glut5; Slc5a; AI526984
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Glucose Transporter 5 GLUT5, Antibody; Polyclonal Anti-SLC2A5 Antibody; Background: GLUT5(Glucose transporter 5), also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.; anti-SLC2A5 antibody
Ordering
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
501
Applicable Applications for anti-SLC2A5 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Glucose Transporter 5 GLUT5 (232-251aa ALQTLRGWKDVHLEMEEIRK), different from the related rat sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of GLUT5 using anti- GLUT5 antibodyGLUT5 was detected in paraffin-embedded section of mouse ggj3249 tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- GLUT5 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

product-image-AAA44918_IHC10.jpg IHC (Immunohistochemistry) (Figure 4. IHC analysis of GLUT5 using anti- GLUT5 antibodyGLUT5 was detected in paraffin-embedded section of mouse ggj3249 tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- GLUT5 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemisry)

(Anti-SLC2A5 antibody, IHC(F)IHC(F): Rat Intestine Tissue)

product-image-AAA44918_IHC11.jpg IHC (Immunohistochemisry) (Anti-SLC2A5 antibody, IHC(F)IHC(F): Rat Intestine Tissue)

IHC (Immunohiostchemistry)

(Anti-SLC2A5 antibody, IHC(P)IHC(P): Rat Kidney Tissue)

product-image-AAA44918_IHC13.jpg IHC (Immunohiostchemistry) (Anti-SLC2A5 antibody, IHC(P)IHC(P): Rat Kidney Tissue)

WB (Western Blot)

(Anti-SLC2A5 antibody, PA1737, Western blottingAll lanes: Anti SLC2A5 at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 55KDObserved bind size: 55KD)

product-image-AAA44918_WB15.jpg WB (Western Blot) (Anti-SLC2A5 antibody, PA1737, Western blottingAll lanes: Anti SLC2A5 at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 55KDObserved bind size: 55KD)
Related Product Information for anti-SLC2A5 antibody
Background: GLUT5(Glucose transporter 5), also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Solute carrier family 2, facilitated glucose transporter member 5
NCBI Official Synonym Full Names
solute carrier family 2 (facilitated glucose transporter), member 5
NCBI Official Symbol
Slc2a5
NCBI Official Synonym Symbols
Glut5; Slc5a; AI526984
NCBI Protein Information
solute carrier family 2, facilitated glucose transporter member 5; GLUT-5; fructose transporter; glucose transporter type 5, small intestine
UniProt Protein Name
Solute carrier family 2, facilitated glucose transporter member 5
UniProt Gene Name
Slc2a5
UniProt Synonym Gene Names
Glut5; GLUT-5
UniProt Entry Name
GTR5_MOUSE

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Product Notes

The SLC2A5 slc2a5 (Catalog #AAA44918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Anti-SLC2A5 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glucose Transporter 5 GLUT5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SLC2A5 slc2a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucose Transporter 5 GLUT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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