WB (Western Blot)
(Western blot analysis of Phospho-Glycogen Synthase (Tyr44) in lysates of NIH-3T3 , using Phospho-Glycogen Synthase (Tyr44) Antibody.)
Rabbit Glycogen Synthase Polyclonal Antibody | anti-GYS antibody
Phospho-Glycogen Synthase (Tyr44) Antibody
Gene Names
GYS1; GSY; GYS
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
Glycogen Synthase, Antibody; Phospho-Glycogen Synthase (Tyr44) Antibody; Glycogen [starch] synthase; Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; Gys1; GYS1_HUMAN; muscle; anti-GYS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-Glycogen Synthase (Tyr44) Antibody detects endogenous levels of Glycogen Synthase only when phosphorylated at Tyr44.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
673
Applicable Applications for anti-GYS antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Glycogen Synthase around the phosphorylation site of Tyr44.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-Glycogen Synthase (Tyr44) in lysates of NIH-3T3 , using Phospho-Glycogen Synthase (Tyr44) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-Glycogen Synthase (Tyr44) in lysates of NIH-3T3 , using Phospho-Glycogen Synthase (Tyr44) Antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-GYS antibody
Description: Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Post Translational Modifications: Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Subunit Structure: Interacts with GYG1.
Similarity: Belongs to the glycosyltransferase 3 family.
Post Translational Modifications: Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Subunit Structure: Interacts with GYG1.
Similarity: Belongs to the glycosyltransferase 3 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 85 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
glycogen
NCBI Official Synonym Full Names
glycogen synthase 1
NCBI Official Symbol
GYS1
NCBI Official Synonym Symbols
GSY; GYS
NCBI Protein Information
glycogen [starch] synthase, muscle
UniProt Protein Name
Glycogen [starch] synthase, muscle
UniProt Gene Name
GYS1
UniProt Synonym Gene Names
GYS
UniProt Entry Name
GYS1_HUMAN
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Product Notes
The GYS gys1 (Catalog #AAA328383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Glycogen Synthase (Tyr44) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Glycogen Synthase can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GYS gys1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycogen Synthase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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