Application Data
(ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-HRP.)
Goat Anti-Human IgG (gamma chain specific) Secondary Antibody
Goat Anti-Human IgG-HRP
ELISA
HRP conjugate: 1:4,000-1:8,000
Application Data
(ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-HRP.)
Application Data
(ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-HRP.)
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Product Notes
The Goat Anti-Human IgG (gamma chain specific) (Catalog #AAA14904) is a Secondary Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Goat Anti-Human IgG (gamma chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA. Research Application(s): ELISA HRP conjugate: 1:4,000-1:8,000. Researchers should empirically determine the suitability of the Goat Anti-Human IgG (gamma chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is goat anti-human IgG?
Goat anti-human IgG is a polyclonal secondary antibody produced by immunizing goats with human IgG protein. The resulting serum undergoes IgG fraction purification and affinity chromatography to isolate antibodies specifically recognizing human immunoglobulin G molecules, creating a multi-epitope detection reagent for immunoassay formats.
What is an anti-human IgG antibody used for?
Anti-human IgG secondary antibodies detect and quantify human IgG primary antibodies in multiple formats: ELISA (capturing or detecting human IgG), Western blotting (HRP-conjugated versions), immunofluorescence (fluorophore-conjugated), immunoprecipitation, and flow cytometry. This antibody bridges between human primary antibodies and detection systems.
Why is goat anti-human IgG used as a secondary antibody?
Goat anti-human IgG is preferred because: (1) goat sera provide high-titer, specific antibodies with minimal cross-reactivity; (2) goat is phylogenetically distant, reducing background in mammalian experiments; (3) the polyclonal nature ensures broad human IgG detection across all subclasses (IgG1-4); (4) goat anti-human antibodies are commercially well-established with extensive validation.
What does an anti-IgG antibody detect?
An anti-IgG antibody specifically detects the constant (Fc) region and hypervariable regions of IgG molecules. This antibody recognizes epitopes on the three constant domains (CH1, CH2, CH3) common to all human IgG molecules and subclasses. When conjugated to enzymes or fluorophores, it enables quantitative detection of IgG concentration or IgG-bound antigens.
What is the difference between anti-human IgG and anti-goat IgG?
Anti-human IgG detects human IgG molecules (primary antibodies from human origin); anti-goat IgG detects goat IgG molecules (secondary antibodies produced in goats). Anti-human IgG serves as a secondary reagent detecting human primary antibodies, while anti-goat IgG would be tertiary, detecting goat-derived secondaries. They target different host species in the immunoassay hierarchy.
Can goat anti-human IgG be used in ELISA or Western blot?
Yes, this antibody is highly suitable for both applications. In ELISA, it functions as a capture or detection antibody in sandwich formats, providing sensitive quantification of human IgG-coated antigens. In Western blot, HRP-conjugated versions detect human IgG primary antibodies with a strong signal and minimal background across all IgG subclasses.
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