Rabbit GPAT1 Polyclonal Antibody | anti-GPAM antibody

GPAT1 Antibody

Cat. Num. AAA39300

• Gene Names: GPAM; GPAT; GPAT1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: GPAT1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: GPAT1, Antibody; GPAT1 Antibody; GPAT1; GPAT; KIAA1560; Glycerol-3-phosphate acyltransferase 1; mitochondrial; GPAT-1; glycerol-3-phosphate acyltransferase, mitochondrial; anti-GPAM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.
• Purity/Purification: GPAT1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 828

• Applicable Applications for anti-GPAM antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1.The immunogen is located within amino acids 730 - 780 of GPAT1.
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%)
• Preparation and Storage: GPAT1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μg/mL.)

IHC (Immunohistochemisry)

(Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μg/ml.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-GPAM antibody

GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 190358539
• NCBI GeneID: 57678
• NCBI Accession #: NP_065969
• NCBI GenBank Nucleotide #: NM_020918.5
• UniProt Accession #: Q9HCL2; Q5VW51; Q86TA3
• Molecular Weight: Predicted: 87, 91 kDa; Observed: 92 kDa
• NCBI Official Full Name: glycerol-3-phosphate acyltransferase 1, mitochondrial
• NCBI Official Synonym Full Names: glycerol-3-phosphate acyltransferase, mitochondrial
• NCBI Official Symbol: GPAM
• NCBI Official Synonym Symbols: GPAT; GPAT1
• NCBI Protein Information: glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT-1; glycerol 3-phosphate acyltransferase, mitochondrial
• UniProt Protein Name: Glycerol-3-phosphate acyltransferase 1, mitochondrial
• UniProt Gene Name: GPAM
• UniProt Synonym Gene Names: GPAT1; KIAA1560; GPAT-1
• UniProt Entry Name: GPAT1_HUMAN