WB (Western Blot)
(Spinal cord (Mouse) Lysate at 40 ug. Primary: Anti-GPCR EX33 at 1/300 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 44 kD. Observed band size: 44kD.)
Rabbit GPCR EX33 Polyclonal Antibody | anti-GPCREX33 antibody
GPCR EX33
Reactivity
Human, Mouse, Pig, Cow, Rabbit
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A affinity purified.
Synonyms
GPCR EX33, Antibody; GPCR EX33; EX33; G-protein coupled receptor 84; GPCR4; GPR84; Inflammation-related G-protein coupled receptor EX33; GPR84_HUMAN; anti-GPCREX33 antibody
Host
Rabbit
Reactivity
Human, Mouse, Pig, Cow, Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A affinity purified.
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-GPCREX33 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from human GPCR EX33 protein 21-120/369
Conjugation
APC
Positive control
Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart,muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B lymphocytes.
Subcellular location
Cell membrane; Multi-pass membrane protein.
Preparation and Storage
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.
WB (Western Blot)
(Spinal cord (Mouse) Lysate at 40 ug. Primary: Anti-GPCR EX33 at 1/300 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 44 kD. Observed band size: 44kD.)
WB (Western Blot)
(Spinal cord (Mouse) Lysate at 40 ug. Primary: Anti-GPCR EX33 at 1/300 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 44 kD. Observed band size: 44kD.)
FCM/FACS (Flow Cytometry)
(Blank control(blue): Mouse thymus cells(fixed with 2% paraformaldehyde (10 min).Primary Antibody: Rabbit Anti- GPCR EX33 antibody, Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
FCM/FACS (Flow Cytometry)
(Blank control(blue): Mouse thymus cells(fixed with 2% paraformaldehyde (10 min).Primary Antibody: Rabbit Anti- GPCR EX33 antibody, Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
IHC (Immunohistochemistry)
(Tissue/cell: rabbit thymus tissue; 4% Paraformaldehyde-fixed and paraffinembedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 ? for 20 min; Incubation: Anti-EX33 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C , followed by conjugation to the secondary antibody and DAB staining.)
IHC (Immunohistochemistry)
(Tissue/cell: rabbit thymus tissue; 4% Paraformaldehyde-fixed and paraffinembedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 ? for 20 min; Incubation: Anti-EX33 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C , followed by conjugation to the secondary antibody and DAB staining.)
Related Product Information for anti-GPCREX33 antibody
Description: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs.Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
44 KDa
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Product Notes
The GPCREX33 (Catalog #AAA268997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPCR EX33 reacts with Human, Mouse, Pig, Cow, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's GPCR EX33 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GPCREX33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPCR EX33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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