WB (Western Blot)
(Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody AAA212775 (lot AAA212775-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse GRASP65 Polyclonal Antibody | anti-GRASP65 antibody
Rabbit anti-GRASP65 Antibody, Affinity Purified
Gene Names
GORASP1; P65; GOLPH5; GRASP65
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GRASP65, Antibody; Rabbit anti-GRASP65 Antibody, Affinity Purified; golgi reassembly-stacking protein of 65 kDa; P65; GOLPH5; Golgi reassembly-stacking protein 1; golgi reassembly stacking protein 1, 65kDa; Golgi reassembly and stacking protein 1; Golgi phosphoprotein 5; Golgi peripheral membrane protein p65; GRASP65; golgi reassembly stacking protein 1; anti-GRASP65 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GRASP65 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 390 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody AAA212775 (lot AAA212775-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody AAA212775 (lot AAA212775-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GRASP65 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody AAA212775 (lot AAA212775-1) used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody For blotting immunoprecipitated GRASP65, AAA212775 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GRASP65 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody AAA212775 (lot AAA212775-1) used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody For blotting immunoprecipitated GRASP65, AAA212775 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-GRASP65 antibody
Golgi reassembly-stacking protein of 65 kDa (GRASP65) is a stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. GRASP65 is a key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. It also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BQQ3].
Product Categories/Family for anti-GRASP65 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,237 Da
NCBI Official Full Name
Golgi reassembly-stacking protein 1 isoform 1
NCBI Official Synonym Full Names
golgi reassembly stacking protein 1, 65kDa
NCBI Official Symbol
GORASP1
NCBI Official Synonym Symbols
P65; GOLPH5; GRASP65
NCBI Protein Information
Golgi reassembly-stacking protein 1; Golgi phosphoprotein 5; Golgi peripheral membrane protein p65; Golgi reassembly and stacking protein 1; golgi reassembly-stacking protein of 65 kDa
UniProt Protein Name
Golgi reassembly-stacking protein 1
UniProt Gene Name
GORASP1
UniProt Synonym Gene Names
GOLPH5; GRASP65; GOLPH5; GRASP65
UniProt Entry Name
GORS1_HUMAN
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Product Notes
The GRASP65 gorasp1 (Catalog #AAA212775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GRASP65 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GRASP65 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GRASP65 gorasp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRASP65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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