Rabbit anti-Human GRB2 Polyclonal Antibody | anti-GRB2 antibody

Anti-GRB2 Antibody Picoband

Cat. Num. AAA126013

• Gene Names: GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: GRB2, Antibody; Anti-GRB2 Antibody Picoband; anti-GRB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-GRB2 antibody: ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human GRB2 recombinant protein (Position: M1-V217).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-GRB2 antibody (AAA126013).Overlay histogram showing PC-3 cells stained with AAA126013 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB2 Antibody (AAA126013, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot)

(Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (AAA126013).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human THP-1 whole cell lysates,Lane 3: human HL-60 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (#AAA126013) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25 kDa. The expected band size for GRB2 is at 25 kDa.)

Related Product Information for anti-GRB2 antibody

GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-GRB2 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4504111
• NCBI GeneID: 2885
• NCBI Accession #: NP_002077.1
• NCBI GenBank Nucleotide #: NM_002086.4
• UniProt Accession #: P62993; P29354; Q14450; Q63057; Q63059
• Molecular Weight: 25,206 Da
• NCBI Official Full Name: growth factor receptor-bound protein 2 isoform 1
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 2
• NCBI Official Symbol: GRB2
• NCBI Official Synonym Symbols: ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
• NCBI Protein Information: growth factor receptor-bound protein 2; HT027; protein Ash; SH2/SH3 adapter GRB2; abundant SRC homology; growth factor receptor-bound protein 3; epidermal growth factor receptor-binding protein GRB2
• UniProt Protein Name: Growth factor receptor-bound protein 2
• UniProt Gene Name: GRB2
• UniProt Synonym Gene Names: ASH
• UniProt Entry Name: GRB2_HUMAN