WB (Western Blot)
Rabbit GRP78 (Bip) Polyclonal Antibody | anti-Hspa5 antibody
GRP78 Antibody: RPE
Gene Names
Hspa5; BIP; GRP78
Reactivity
Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus
Applications
Immunofluorescence, Western Blot
Synonyms
GRP78 (Bip), Antibody; GRP78 Antibody: RPE; BIP; Grp78; HspA5; MIF2; immunoglobulin heavy chain binding protein; anti-Hspa5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus
Clonality
Polyclonal
Form/Format
Rabbit Antiserum
Sequence Length
654
Applicable Applications for anti-Hspa5 antibody
IF (Immunofluorescence), WB (Western Blot)
Conjugate
RPE
Storage Buffer
Rabbit antiserum
Preparation and Storage
-20 degree C
WB (Western Blot)
WB (Western Blot)
WB (Western Blot)
WB (Western Blot)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Tissue: Hippocampal Section. Species: Mouse. Fixation: 4% Formaldehyde for 12 hours at RT. Paraffin embedded. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Rabbit at 1:250 for 1 hour at RT. Counterstain: Hoechst at 1:1000 for 10 min at RT. Localization: Grp78 staining in mouse pyramidal cell layer. Magnification: 20x. Slice thickness: 7 microns)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Tissue: Hippocampal Section. Species: Mouse. Fixation: 4% Formaldehyde for 12 hours at RT. Paraffin embedded. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Rabbit at 1:250 for 1 hour at RT. Counterstain: Hoechst at 1:1000 for 10 min at RT. Localization: Grp78 staining in mouse pyramidal cell layer. Magnification: 20x. Slice thickness: 7 microns)
Related Product Information for anti-Hspa5 antibody
Background Info: Detects a protein with a mass of ~78kDa corresponding to Grp78.
Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Product Categories/Family for anti-Hspa5 antibody
References
1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,347 Da
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock protein 5
NCBI Official Symbol
Hspa5
NCBI Official Synonym Symbols
BIP; GRP78
NCBI Protein Information
78 kDa glucose-regulated protein; GRP 78; GRP-78; heat shock 70kD protein 5; heat shock 70 kDa protein 5; steroidogenesis-activator polypeptide; immunoglobulin heavy chain-binding protein; heat shock 70kDa protein 5 (glucose-regulated protein)
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
Hspa5
UniProt Synonym Gene Names
Grp78; GRP-78; BiP
UniProt Entry Name
GRP78_RAT
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Product Notes
The Hspa5 hspa5 (Catalog #AAA103517) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GRP78 Antibody: RPE reacts with Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's GRP78 (Bip) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Hspa5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP78 (Bip), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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