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IP (Immunoprecipitation)
(HSPA5 was immunoprecipitated using:Lane A:0.5 mg HL60 Whole Cell Lysate1µL anti-HSPA5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HSPA5 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 72 kDaObserved band size :82 kDa)
Rabbit GRP78/HSPA5 Polyclonal Antibody | anti-GRP78/HSPA5 antibody
Anti-GRP78/HSPA5 Antibody, Rabbit Polyclonal
Reactivity
Human, Mouse, Rat(Species predicted to react based on 100% sequence homology)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
GRP78/HSPA5, Antibody; Anti-GRP78/HSPA5 Antibody, Rabbit Polyclonal; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Anti-BIP Antibody; Anti-GRP78 Antibody; Anti-HEL-S-89n Antibody; Anti-MIF2 Antibody; anti-GRP78/HSPA5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GRP78/HSPA5 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:2500-1:10000
IP: 0.2-1uL/mg of lysate
IHC-P: 1:2500-1:10000
IP: 0.2-1uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human HSPA5
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human HSPA5, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(HSPA5 was immunoprecipitated using:Lane A:0.5 mg HL60 Whole Cell Lysate1µL anti-HSPA5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HSPA5 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 72 kDaObserved band size :82 kDa)
IP (Immunoprecipitation)
(HSPA5 was immunoprecipitated using:Lane A:0.5 mg HL60 Whole Cell Lysate1µL anti-HSPA5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HSPA5 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 72 kDaObserved band size :82 kDa)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in rat testis with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in rat testis with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in mouse prostate with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in mouse prostate with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in human testis with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in human testis with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in human prostatic carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining HSPA5 in human prostatic carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-HSPA5 rabbit polyclonal antibody at 1:500 dilutionLane A: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:72 kDaObserved band size:72 kDa)
WB (Western Blot)
(Anti-HSPA5 rabbit polyclonal antibody at 1:500 dilutionLane A: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:72 kDaObserved band size:72 kDa)
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Product Notes
The GRP78/HSPA5 (Catalog #AAA27773) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRP78/HSPA5 Antibody, Rabbit Polyclonal reacts with Human, Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's GRP78/HSPA5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC-P: 1:2500-1:10000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the GRP78/HSPA5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP78/HSPA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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