WB (Western Blot)
(GSK3A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GSK3A antibody detected the GSK3A protein (arrow).)
Rabbit anti-Human GSK3 alpha (GSK3A) Polyclonal Antibody | anti-GSK3A antibody
GSK3 alpha (GSK3A) Antibody (C-term)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GSK3 alpha (GSK3A), Antibody; GSK3 alpha (GSK3A) Antibody (C-term); Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; anti-GSK3A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GSK3 alpha (GSK3A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 448-478 amino acids from the C-terminal region of human GSK3 alpha (GSK3A).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
448-478
Sequence Length
483
Applicable Applications for anti-GSK3A antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(GSK3A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GSK3A antibody detected the GSK3A protein (arrow).)
WB (Western Blot)
(GSK3A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GSK3A antibody detected the GSK3A protein (arrow).)
WB (Western Blot)
(Western blot analysis of GSK3A (arrow) using GSK3A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GSK3A gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of GSK3A (arrow) using GSK3A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GSK3A gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-GSK3A antibody
Glycogen synthase kinase 3-alpha (GSK3A)is a multifunctional protein serine kinase implicated in the control of several regulatory proteins including glycogen synthase and transcription factors. It also plays a role in the WNT and PI3K signaling pathways.1 Under resting conditions GSK3A and its homologs are highly phosphorylated at tyr279 in the phosphorylation loop.2 Constitutive phosphorylation of this tyrosine is important for kinase activity. Dephosphorylation of tyr279 after mitogen activation is accompanied by kinase inactivation. PKA as well as PI3K-activated PKB inactivate GSK3A by phosphorylation at ser21.3 Lysophosphatidic acid primarily utilizes a PKC-dependent pathway to modulate GSK3 and certain growth factors (e.g., PDGFB), which control GSK3 mainly through PIK3-PKB, are able to regulate GSK3 through an alternative, redundant phospholipase-C-gamma-PKC pathway.4 Alzheimer disease (AD) is associated with increased production and aggregation of amyloid-beta-40 and -42 peptides into plaques. GSK3A is required for maximal production of the beta-amyloid-40 and -42 peptides generated from the amyloid precursor protein (APP) by presenilin (PSEN1)-dependent gamma-secretase cleavage.5 In vitro, lithium, a GSK3A inhibitor, blocked the production of the beta-amyloid peptides by interfering with the gamma-secretase step. In mice expressing familial AD-associated mutations in APP and PSEN1, lithium reduced the levels of beta-amyloid peptides GSK3A also phosphorylates the tau protein (MAPT), the principal component of neurofibrillary tangles in AD, and suggested that inhibition of GSK3A may offer a new therapeutic approach to AD.
Product Categories/Family for anti-GSK3A antibody
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50981
NCBI Official Full Name
glycogen synthase kinase-3 alpha
NCBI Official Synonym Full Names
glycogen synthase kinase 3 alpha
NCBI Official Symbol
GSK3A
NCBI Protein Information
glycogen synthase kinase-3 alpha
UniProt Protein Name
Glycogen synthase kinase-3 alpha
UniProt Gene Name
GSK3A
UniProt Synonym Gene Names
GSK-3 alpha
UniProt Entry Name
GSK3A_HUMAN
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Product Notes
The GSK3A gsk3a (Catalog #AAA284395) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 448-478. The GSK3 alpha (GSK3A) Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3 alpha (GSK3A) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GSK3A gsk3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3 alpha (GSK3A), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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