Rabbit GSK3alpha Polyclonal Antibody | anti-GSK3alpha antibody

GSK3alpha (Phospho-Ser21) Antibody

Cat. Num. AAA304955

• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: GSK3alpha, Antibody; GSK3alpha (Phospho-Ser21) Antibody; GSK3a (Phospho-Ser21) Antibody; Factor A; GSK-3 alpha; kinase GSK3-alpha; anti-GSK3alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21 and does not detect GSK-3b when phosphorylated at Ser9.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-GSK3alpha antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.
• Modification: Phospho-Ser21
• Target Name: GSK3a
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

WB (Western Blot)

(Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3alpha (Phospho-Ser21) Antibody.)

IF (Immunofluorescence)

(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3a(Phospho-Ser21) Antibody.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a(Phospho-Ser21) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot)

(Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3a(Phospho-Ser21) Antibody.)

Related Product Information for anti-GSK3alpha antibody

Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.

Product Categories/Family for anti-GSK3alpha antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha
• UniProt Entry Name: GSK3A_HUMAN