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IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MEF cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
Rabbit GSK3alpha/beta Polyclonal Antibody | anti-GSK3alpha/beta antibody
GSK3alpha/beta(Phospho-Tyr279/216) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
GSK3alpha/beta, Antibody; GSK3alpha/beta(Phospho-Tyr279/216) Antibody; GSK3a/b(Phospho-Tyr279/216) Antibody; Factor A; GSK-3 alpha/beta; kinase GSK3-alpha/beta; anti-GSK3alpha/beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of GSK3alpha/beta only when phosphorylated at tyrosine 279/216.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-GSK3alpha/beta antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3alpha/beta.
Modification
Phospho-Tyr279/216
Target Name
GSK3alpha/beta
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MEF cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MEF cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3alpha/beta(Phospho-Tyr279/216) Antibody (left) or the same antibody preincubated with blocking peptide #51301 (right).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3alpha/beta(Phospho-Tyr279/216) Antibody (left) or the same antibody preincubated with blocking peptide #51301 (right).)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)
Related Product Information for anti-GSK3alpha/beta antibody
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Product Categories/Family for anti-GSK3alpha/beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,981 Da
NCBI Official Full Name
glycogen synthase kinase-3 alpha
NCBI Official Synonym Full Names
glycogen synthase kinase 3 alpha
NCBI Official Symbol
GSK3A
NCBI Protein Information
glycogen synthase kinase-3 alpha
UniProt Protein Name
Glycogen synthase kinase-3 alpha
UniProt Gene Name
GSK3A
UniProt Synonym Gene Names
GSK-3 alpha
UniProt Entry Name
GSK3A_HUMAN
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Product Notes
The GSK3alpha/beta gsk3a (Catalog #AAA305473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSK3alpha/beta(Phospho-Tyr279/216) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3alpha/beta can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GSK3alpha/beta gsk3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3alpha/beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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