<
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3beta (Phospho-Ser9) Antibody.)
Rabbit GSK3beta Polyclonal Antibody | anti-GSK3beta antibody
GSK3beta (Phospho-Ser9) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
GSK3beta; Polyclonal Antibody; GSK3beta (Phospho-Ser9) Antibody; GSK3b (Phospho-Ser9) Antibody; Factor A; GSK-3 beta; Protein kinase GSK-3-beta; kinase GSK-3 beta; anti-GSK3beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
420
Applicable Applications for anti-GSK3beta antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3beta.
Modification
Phospho-Ser9
Target Name
GSK3beta
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3beta (Phospho-Ser9) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3beta (Phospho-Ser9) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3beta (Phospho-Ser9) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3beta (Phospho-Ser9) Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3beta (Phospho-Ser9) Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3beta (Phospho-Ser9) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3beta(Phospho-Ser9) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3beta(Phospho-Ser9) Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3beta (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide #51002 (right).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3beta (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide #51002 (right).)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3beta(Phospho-Ser9) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3beta(Phospho-Ser9) Antibody.)
Related Product Information for anti-GSK3beta antibody
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Product Categories/Family for anti-GSK3beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,034 Da
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 2
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
UniProt Entry Name
GSK3B_HUMAN
Similar Products
Product Notes
The GSK3beta gsk3b (Catalog #AAA29709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSK3beta (Phospho-Ser9) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200. Researchers should empirically determine the suitability of the GSK3beta gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
