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FCM/FACS (Flow Cytometry)
(GSN Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit GSN Polyclonal Antibody | anti-GSN antibody
GSN Antibody (N-term)
Gene Names
GSN; ADF; AGEL
Reactivity
Human, Mouse, Rat; Predicted: Bovine
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
GSN, Antibody; GSN Antibody (N-term); Gelsolin; AGEL; Actin-depolymerizing factor; ADF; Brevin; GSN; anti-GSN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat; Predicted: Bovine
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the N-terminal region of human GSN.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
230-259
Applicable Applications for anti-GSN antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Clone Names
RB18721
Function
Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Cellular Location
Isoform 2: Cytoplasm, cytoskeleton
Tissue Location
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells
Preparation and Storage
Maintain refrigerated at 2-8° C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GSN Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GSN Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(GSN Antibody (N-term) (RB18721) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(GSN Antibody (N-term) (RB18721) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-GSN Antibody (N-term) at 1:1000-1:2000 dilutionLane 1: human plasma lysatesLane 2: Hela whole cell lysatesLane 3: mouse spleen lysatesLane 4: rat kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-GSN Antibody (N-term) at 1:1000-1:2000 dilutionLane 1: human plasma lysatesLane 2: Hela whole cell lysatesLane 3: mouse spleen lysatesLane 4: rat kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-GSN Antibody (N-term)at 1:2000 dilution + MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-GSN Antibody (N-term)at 1:2000 dilution + MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GSN antibody
GSN binds to the 'plus' ends of actin monomers and filaments to prevent monomer exchange. The calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this protein are a cause of familial amyloidosis Finnish type (FAF).
Product Categories/Family for anti-GSN antibody
References
Li,Q., Ye,Z. Biochem. Biophys. Res. Commun. 385 (2), 284-289 (2009) Walsh,N., Dowling,P. J Proteomics 71 (5), 561-571 (2008) Paunio,T., Kiuru,S. Genomics 13 (1), 237-239 (1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85698
NCBI Official Full Name
gelsolin isoform a
NCBI Official Synonym Full Names
gelsolin
NCBI Official Symbol
GSN
NCBI Official Synonym Symbols
ADF; AGEL
NCBI Protein Information
gelsolin
UniProt Protein Name
Gelsolin
UniProt Gene Name
GSN
UniProt Synonym Gene Names
ADF
UniProt Entry Name
GELS_HUMAN
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Product Notes
The GSN gsn (Catalog #AAA290532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 230-259. The GSN Antibody (N-term) reacts with Human, Mouse, Rat; Predicted: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's GSN can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GSN gsn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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