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IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Immunohistochemistry of paraffin-embedded rat kidney using GSS antibody at dilution of 1:200 (x400 lens))
Rabbit anti-Mouse, Human GSS/Glutathione Synthetase Polyclonal Antibody | anti-GSS antibody
GSS/Glutathione Synthetase Rabbit anti-Human Polyclonal Antibody
Gene Names
GSS; GSHS; HEL-S-64p; HEL-S-88n
Reactivity
Mouse, Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Synonyms
GSS/Glutathione Synthetase; Polyclonal Antibody; GSS/Glutathione Synthetase Rabbit anti-Human Polyclonal Antibody; IHC-plus GSS/Glutathione Synthetase Antibody; GSS; GSH-S; GSHS; Glutathione synthase; Glutathione synthetase; GSH synthetase; anti-GSS antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human GSS/Glutathione Synthetase
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol
Applicable Applications for anti-GSS antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Application Notes
IF: 1:50-1:200
IHC: 1:50-1:200
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 40kDa/52kDa, while the observed MW by Western blot was 53kDa.
IHC: 1:50-1:200
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 40kDa/52kDa, while the observed MW by Western blot was 53kDa.
Target
Human GSS/Glutathione Synthetase
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human GSS (NP_000169.1). MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKD
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Immunohistochemistry of paraffin-embedded rat kidney using GSS antibody at dilution of 1:200 (x400 lens))
IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Immunohistochemistry of paraffin-embedded rat kidney using GSS antibody at dilution of 1:200 (x400 lens))
IHC (Immunohistchemistry)
(GSS/Glutathione Synthetase Antibody-Immunohistochemistry of paraffin-embedded mouse lung tissue.)
IHC (Immunohistchemistry)
(GSS/Glutathione Synthetase Antibody-Immunohistochemistry of paraffin-embedded mouse lung tissue.)
IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(GSS/Glutathione Synthetase Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(GSS/Glutathione Synthetase Antibody-Western blot analysis of extracts of various cell lines, using GSS antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)
WB (Western Blot)
(GSS/Glutathione Synthetase Antibody-Western blot analysis of extracts of various cell lines, using GSS antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)
ICC (Immunocytochemistry)
(GSS/Glutathione Synthetase Antibody-Immunofluorescence analysis of A549 cells.)
ICC (Immunocytochemistry)
(GSS/Glutathione Synthetase Antibody-Immunofluorescence analysis of A549 cells.)
ICC (Immunocytochemistry)
(GSS/Glutathione Synthetase Antibody-Immunofluorescence analysis of HeLa cells using GSS antibody. Blue: DAPI for nuclear staining.)
ICC (Immunocytochemistry)
(GSS/Glutathione Synthetase Antibody-Immunofluorescence analysis of HeLa cells using GSS antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-GSS antibody
Glutathione Synthetase antibody is an unconjugated rabbit polyclonal antibody to Glutathione Synthetase (GSS) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,349 Da
NCBI Official Full Name
glutathione synthetase
NCBI Official Synonym Full Names
glutathione synthetase
NCBI Official Symbol
GSS
NCBI Official Synonym Symbols
GSHS; HEL-S-64p; HEL-S-88n
NCBI Protein Information
glutathione synthetase; GSH synthetase; GSH-S; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n; glutathione synthase
UniProt Protein Name
Glutathione synthetase
UniProt Gene Name
GSS
UniProt Synonym Gene Names
GSH synthetase; GSH-S
UniProt Entry Name
GSHB_HUMAN
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Product Notes
The GSS gss (Catalog #AAA21300) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSS/Glutathione Synthetase Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSS/Glutathione Synthetase can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). IF: 1:50-1:200 IHC: 1:50-1:200 IHC-P: 1:200 WB: 1:500-1:2000 The predicted MW is 40kDa/52kDa, while the observed MW by Western blot was 53kDa. Researchers should empirically determine the suitability of the GSS gss for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSS/Glutathione Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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