WB (Western Blot)
(WB Suggested Anti-H2AFX Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: NCI-H226 cell lysateThere is BioGPS gene expression data showing that H2AFX is expressed in NCIH226)
Rabbit H2AFX Polyclonal Antibody | anti-H2AFX antibody
H2AFX antibody - middle region
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Synonyms
H2AFX, Antibody; H2AFX antibody - middle region; anti-H2AFX antibody
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: ELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQE
Sequence Length
143
Applicable Applications for anti-H2AFX antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
Homology
Cow: 93%; Dog: 93%; Guinea Pig: 93%; Human: 100%; Mouse: 79%; Rat: 86%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human H2AFX
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-H2AFX Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: NCI-H226 cell lysateThere is BioGPS gene expression data showing that H2AFX is expressed in NCIH226)
WB (Western Blot)
(WB Suggested Anti-H2AFX Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: NCI-H226 cell lysateThere is BioGPS gene expression data showing that H2AFX is expressed in NCIH226)
IHC (Immunohiostchemistry)
(Immunohistochemistry with Tonsil tissue at an antibody concentration of 5ug/ml using anti-H2AFX antibody)
IHC (Immunohiostchemistry)
(Immunohistochemistry with Tonsil tissue at an antibody concentration of 5ug/ml using anti-H2AFX antibody)
IF (Immunofluorescence)
(Sample Type :MCF7Primary Antibody Dilution:4 ug/mlSecondary Antibody :Anti-rabbit Alexa 546Secondary Antibody Dilution:2 ug/mlGene Name :H2AFX)
IF (Immunofluorescence)
(Sample Type :MCF7Primary Antibody Dilution:4 ug/mlSecondary Antibody :Anti-rabbit Alexa 546Secondary Antibody Dilution:2 ug/mlGene Name :H2AFX)
Related Product Information for anti-H2AFX antibody
This is a rabbit polyclonal antibody against H2AFX. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA
Target Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA
Product Categories/Family for anti-H2AFX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16kDa
NCBI Official Full Name
histone H2AX
NCBI Official Synonym Full Names
H2A histone family member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2AX
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2AFX h2afx (Catalog #AAA200798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H2AFX antibody - middle region reacts with Cow, Dog, Guinea Pig, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's H2AFX can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the H2AFX h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: ELNKLLGGVT IAQGGVLPNI QAVLLPKKTS ATVGPKAPSG GKKATQASQE. It is sometimes possible for the material contained within the vial of "H2AFX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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