IHC (Immunohistochemisry)
(H2AFZ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of H2AFZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit H2AFZ Polyclonal Antibody | anti-H2AFZ antibody
H2AFZ Antibody (C-term)
Gene Names
H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
Reactivity
Human (Predicted Reactivity: Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Xenopus, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
H2AFZ, Antibody; H2AFZ Antibody (C-term); Histone H2AZ; H2A/z; H2AFZ; H2AZ; anti-H2AFZ antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Xenopus, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This H2AFZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from the C-terminal region of human H2AFZ.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
59-86
Sequence Length
128
Applicable Applications for anti-H2AFZ antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(H2AFZ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of H2AFZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(H2AFZ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of H2AFZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(H2AFZ Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the H2AFZ antibody detected the H2AFZ protein (arrow).)
WB (Western Blot)
(H2AFZ Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the H2AFZ antibody detected the H2AFZ protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysate from zebra fish heart tissue, using H2AFZ Antibody (C-term). AAA283907 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from zebra fish heart tissue, using H2AFZ Antibody (C-term). AAA283907 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-H2AFZ antibody
Histones are basic nuclear proteins that are responsible
for the nucleosome structure of the chromosomal fiber in
eukaryotes. Nucleosomes consist of approximately 146 bp of DNA
wrapped around a histone octamer composed of pairs of each of the
four core histones (H2A, H2B, H3, and H4). The chromatin fiber is
further compacted through the interaction of a linker histone, H1,
with the DNA between the nucleosomes to form higher order chromatin
structures. This gene encodes a replication-independent member of
the histone H2A family that is distinct from other members of the
family. Studies in mice have shown that this particular histone is
required for embryonic development and indicate that lack of
functional histone H2A leads to embryonic lethality. [provided by
RefSeq].
for the nucleosome structure of the chromosomal fiber in
eukaryotes. Nucleosomes consist of approximately 146 bp of DNA
wrapped around a histone octamer composed of pairs of each of the
four core histones (H2A, H2B, H3, and H4). The chromatin fiber is
further compacted through the interaction of a linker histone, H1,
with the DNA between the nucleosomes to form higher order chromatin
structures. This gene encodes a replication-independent member of
the histone H2A family that is distinct from other members of the
family. Studies in mice have shown that this particular histone is
required for embryonic development and indicate that lack of
functional histone H2A leads to embryonic lethality. [provided by
RefSeq].
Product Categories/Family for anti-H2AFZ antibody
References
Marques, M., et al. Epigenetics 5(4):267-272(2010)
Svotelis, A., et al. Cell Cycle 9(2):364-370(2010)
Thakar, A., et al. Biochemistry 48(46):10852-10857(2009)
Hardy, S., et al. PLoS Genet. 5 (10), E1000687 (2009) :
Gevry, N., et al. Genes Dev. 23(13):1522-1533(2009)
Svotelis, A., et al. Cell Cycle 9(2):364-370(2010)
Thakar, A., et al. Biochemistry 48(46):10852-10857(2009)
Hardy, S., et al. PLoS Genet. 5 (10), E1000687 (2009) :
Gevry, N., et al. Genes Dev. 23(13):1522-1533(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13553
NCBI Official Full Name
histone H2A.Z
NCBI Official Synonym Full Names
H2A histone family, member Z
NCBI Official Symbol
H2AFZ
NCBI Official Synonym Symbols
H2AZ; H2A.z; H2A/z; H2A.Z-1
NCBI Protein Information
histone H2A.Z
UniProt Protein Name
Histone H2A.Z
UniProt Gene Name
H2AFZ
UniProt Synonym Gene Names
H2AZ; H2A/z
UniProt Entry Name
H2AZ_HUMAN
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Product Notes
The H2AFZ h2afz (Catalog #AAA283907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 59-86. The H2AFZ Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Xenopus, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's H2AFZ can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the H2AFZ h2afz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H2AFZ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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