IF (Immunofluorescence)
(Immunofluorescence staining of SPINT2-His in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SPINT2-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Rabbit anti-Human HAI-2/SPINT2 Polyclonal Antibody | anti-SPINT2 antibody
Anti-HAI-2/SPINT2 Antibody, Rabbit Polyclonal
Gene Names
SPINT2; PB; Kop; HAI2; DIAR3; HAI-2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
HAI-2/SPINT2, Antibody; Anti-HAI-2/SPINT2 Antibody, Rabbit Polyclonal; SPINT2/Placental bikunin Antibody, Rabbit PAb, Antigen Affinity Purified; serine peptidase inhibitor, Kunitz type, 2; Anti-DIAR3 Antibody; Anti-HAI-2 Antibody; Anti-HAI2 Antibody; Anti-Kop Antibody; Anti-PB Antibody; anti-SPINT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SPINT2/Placental bikunin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SPINT2 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human SPINT2/Placental bikunin Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human SPINT2/Placental bikunin (rh SPINT2/Placental bikunin). SPINT2/Placental bikunin specific IgG was purified by Human SPINT2/Placental bikunin affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of SPINT2-His in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SPINT2-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of SPINT2-His in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SPINT2-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
WB (Western Blot)
(Anti-SPINT2 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Caco2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-SPINT2 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Caco2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-SPINT2 antibody
Choanal (CA) and gastrointestinal atresias (GA) are an important feature of syndromic congenital sodium diarrhea (sCSD), a disorder recently associated with mutations in the gene for serine protease inhibitor type 2 (SPINT2). The SPINT2 gene is epigenetically silenced or downregulated in human cancers, altering the balance of HGF activation/inhibition ratio, which contributes to cancer development and progression. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. Loss of SPINT2 expression in tumors has been associated with gene promoter hypermethylation. SPINT2 (serine peptidase inhibitor Kunitz type 2), a proteolytic inhibitor of hepatocyte growth factor activator (HGFA), has a significant role in the suppression of the HGF-MET pathway and malignant melanoma progression.
Product Categories/Family for anti-SPINT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,828 Da
NCBI Official Full Name
kunitz-type protease inhibitor 2 isoform a
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kunitz type, 2
NCBI Official Symbol
SPINT2
NCBI Official Synonym Symbols
PB; Kop; HAI2; DIAR3; HAI-2
NCBI Protein Information
kunitz-type protease inhibitor 2; placental bikunin; serine protease inhibitor, Kunitz type, 2; hepatocyte growth factor activator inhibitor type 2
UniProt Protein Name
Kunitz-type protease inhibitor 2
UniProt Gene Name
SPINT2
UniProt Synonym Gene Names
HAI2; KOP; HAI-2
UniProt Entry Name
SPIT2_HUMAN
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Product Notes
The SPINT2 spint2 (Catalog #AAA254827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HAI-2/SPINT2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAI-2/SPINT2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SPINT2 spint2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAI-2/SPINT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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