IP (Immunoprecipitation)
(Detection of human Hakai by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Hakai antibody AAA212083 used for IP at 3 ug/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody which recognizes a downstream epitope. For blotting immunoprecipitated Hakai, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human Hakai Polyclonal Antibody | anti-Hakai antibody
Rabbit anti-Hakai Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Hakai, Antibody; Rabbit anti-Hakai Antibody, Affinity Purified; RING-type E3 ubiquitin transferase Hakai; RNF188; RING finger protein 188; HAKAI; E-cadherin binding protein E7; c-Cbl-like protein 1; Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1; Cbl proto-oncogene like 1, E3 ubiquitin protein ligase; Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1; casitas B-lineage lymphoma-transforming sequence-like protein 1; E3 ubiquitin-protein ligase Hakai; Cbl proto-oncogene like 1; anti-Hakai antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Hakai antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Hakai by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Hakai antibody AAA212083 used for IP at 3 ug/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody which recognizes a downstream epitope. For blotting immunoprecipitated Hakai, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Hakai by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Hakai antibody AAA212083 used for IP at 3 ug/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody which recognizes a downstream epitope. For blotting immunoprecipitated Hakai, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Hakai by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Hakai (Cat. No. AAA212083 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Hakai by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Hakai (Cat. No. AAA212083 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Hakai antibody
Hakai is an E3-ubiquitin ligase and an E-cadherin binding protein that plays a role in modulating cell adhesion.
Product Categories/Family for anti-Hakai antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Hakai cbll1 (Catalog #AAA212083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Hakai Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hakai can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Hakai cbll1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hakai, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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