WB (Western Blot)
(HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).)
Rabbit HAND2 Polyclonal Antibody | anti-HAND2 antibody
HAND2 Antibody (Center)
Gene Names
HAND2; Hed; dHand; DHAND2; Thing2; bHLHa26
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Rat, Xenopus)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HAND2, Antibody; HAND2 Antibody (Center); Heart- and neural crest derivatives-expressed protein 2; Class A basic helix-loop-helix protein 26; bHLHa26; Deciduum; heart; autonomic nervous system and neural crest derivatives-expressed protein 2; dHAND; HAND2; BHLHA26; DHAND; anti-HAND2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human HAND2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
81-110
Sequence Length
217
Applicable Applications for anti-HAND2 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).)
WB (Western Blot)
(HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-HAND2 antibody
The protein encoded by this gene belongs to the basic
helix-loop-helix family of transcription factors. This gene product
is one of two closely related family members, the HAND proteins,
which are asymmetrically expressed in the developing ventricular
chambers and play an essential role in cardiac morphogenesis.
Working in a complementary fashion, they function in the formation
of the right ventricle and aortic arch arteries, implicating them
as mediators of congenital heart disease. In addition, this
transcription factor plays an important role in limb and branchial
arch development.
helix-loop-helix family of transcription factors. This gene product
is one of two closely related family members, the HAND proteins,
which are asymmetrically expressed in the developing ventricular
chambers and play an essential role in cardiac morphogenesis.
Working in a complementary fashion, they function in the formation
of the right ventricle and aortic arch arteries, implicating them
as mediators of congenital heart disease. In addition, this
transcription factor plays an important role in limb and branchial
arch development.
Product Categories/Family for anti-HAND2 antibody
References
Shen, L., et al. Chin. Med. J. 123(13):1623-1627(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Voth, H., et al. BMC Mol. Biol. 10, 28 (2009) :
Morikawa, Y., et al. Circ. Res. 103(12):1422-1429(2008)
Han, Z., et al. Development 133(6):1175-1182(2006)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Voth, H., et al. BMC Mol. Biol. 10, 28 (2009) :
Morikawa, Y., et al. Circ. Res. 103(12):1422-1429(2008)
Han, Z., et al. Development 133(6):1175-1182(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23666
NCBI Official Full Name
heart- and neural crest derivatives-expressed protein 2
NCBI Official Synonym Full Names
heart and neural crest derivatives expressed 2
NCBI Official Symbol
HAND2
NCBI Official Synonym Symbols
Hed; dHand; DHAND2; Thing2; bHLHa26
NCBI Protein Information
heart- and neural crest derivatives-expressed protein 2
UniProt Protein Name
Heart- and neural crest derivatives-expressed protein 2
UniProt Gene Name
HAND2
UniProt Synonym Gene Names
BHLHA26; DHAND; bHLHa26; dHAND
UniProt Entry Name
HAND2_HUMAN
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Product Notes
The HAND2 hand2 (Catalog #AAA286908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 81-110. The HAND2 Antibody (Center) reacts with Human (Predicted Reactivity: Chicken, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's HAND2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HAND2 hand2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAND2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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