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WB (Western Blot)
(Western blot analysis of lysates from human brain, mouse brain and eyeball tissue lysate (from left to right), using HBG2 Antibody (C-term). AAA284543 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit anti-Human, mouse HBG2 Polyclonal Antibody | anti-HBG2 antibody
HBG2 Antibody (C-term)
Gene Names
HBG2; TNCY; HBG-T1
Reactivity
Human, mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HBG2, Antibody; HBG2 Antibody (C-term); Hemoglobin subunit gamma-2; Gamma-2-globin; Hb F Ggamma; Hemoglobin gamma-2 chain; Hemoglobin gamma-G chain; HBG2; anti-HBG2 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This HBG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-133 amino acids from the C-terminal region of human HBG2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
147
Applicable Applications for anti-HBG2 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Tissue Location
Red blood cells
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from human brain, mouse brain and eyeball tissue lysate (from left to right), using HBG2 Antibody (C-term). AAA284543 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from human brain, mouse brain and eyeball tissue lysate (from left to right), using HBG2 Antibody (C-term). AAA284543 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody (C-term). AAA284543 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody (C-term). AAA284543 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody (C-term). AAA284543 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody (C-term). AAA284543 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284543 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284543 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-HBG2 antibody
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
Product Categories/Family for anti-HBG2 antibody
References
Slightom J.L.,et al.Cell 21:627-638(1980).
Cavallesco C.,et al.Gene 12:215-221(1980).
Kutlar F.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schroeder W.A.,et al.Biochemistry 2:992-1008(1963).
Cavallesco C.,et al.Gene 12:215-221(1980).
Kutlar F.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schroeder W.A.,et al.Biochemistry 2:992-1008(1963).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hemoglobin subunit gamma-2
NCBI Official Synonym Full Names
hemoglobin, gamma G
NCBI Official Symbol
HBG2
NCBI Official Synonym Symbols
TNCY; HBG-T1
NCBI Protein Information
hemoglobin subunit gamma-2
UniProt Protein Name
Hemoglobin subunit gamma-2
UniProt Gene Name
HBG2
UniProt Entry Name
HBG2_HUMAN
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Product Notes
The HBG2 hbg2 (Catalog #AAA284543) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HBG2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HBG2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HBG2 hbg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HBG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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