IF (Immunofluorescence)
(AAA328557 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA328557 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
Rabbit anti-Human, Mouse HCN1 Polyclonal Antibody | anti-HCN1 antibody
HCN1 Antibody
Gene Names
HCN1; BCNG1; HAC-2; BCNG-1; EIEE24; GEFSP10
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
HCN1, Antibody; HCN1 Antibody; BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC 2; HAC2; HCN1; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1; Potassium channel, voltage-gated, brain, 1; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; anti-HCN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
890
Applicable Applications for anti-HCN1 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human HCN1, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328557 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA328557 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328557 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA328557 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts of Mouse brain tissue sample,using HCN1 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of Mouse brain tissue sample,using HCN1 Antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
99 kDa
NCBI Official Full Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NCBI Official Synonym Full Names
hyperpolarization activated cyclic nucleotide gated potassium channel 1
NCBI Official Symbol
HCN1
NCBI Official Synonym Symbols
BCNG1; HAC-2; BCNG-1; EIEE24; GEFSP10
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Gene Name
HCN1
UniProt Synonym Gene Names
BCNG1; BCNG-1
UniProt Entry Name
HCN1_HUMAN
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Product Notes
The HCN1 hcn1 (Catalog #AAA328557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HCN1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HCN1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the HCN1 hcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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