WB (Western Blot)
(Western blot analysis of extracts of Mouse brain tissue sample,using Phospho-HCN1(Ser846) Antibody.)
Rabbit HCN1 Polyclonal Antibody | anti-HCN1 antibody
Phospho-HCN1 (Ser846) Antibody
Gene Names
HCN1; BCNG1; HAC-2; BCNG-1; EIEE24; GEFSP10
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
HCN1, Antibody; Phospho-HCN1 (Ser846) Antibody; BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC 2; HAC2; HCN1; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1; Potassium channel, voltage-gated, brain, 1; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; anti-HCN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-HCN1(Ser846) Antibody detects endogenous levels of HCN1 only when phosphorylated at Ser846.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
890
Applicable Applications for anti-HCN1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human HCN1 around the phosphorylation site of Ser846.
Tissue Specificity
Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
Subcellular Location
Plasma Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of Mouse brain tissue sample,using Phospho-HCN1(Ser846) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of Mouse brain tissue sample,using Phospho-HCN1(Ser846) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-HCN1 antibody
Description: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions (PubMed:28086084). Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.
Subunit Structure: Homotetramer (PubMed:28086084). Heterotetramer with HCN2. The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits. Interacts with KCNE2. Interacts with the SH3 domain of CSK (By similarity).
Similarity: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Belongs to the potassium channel HCN family.
Subunit Structure: Homotetramer (PubMed:28086084). Heterotetramer with HCN2. The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits. Interacts with KCNE2. Interacts with the SH3 domain of CSK (By similarity).
Similarity: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Belongs to the potassium channel HCN family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 99 kDa
Predicted: 99 kDa
Predicted: 99 kDa
NCBI Official Full Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NCBI Official Synonym Full Names
hyperpolarization activated cyclic nucleotide gated potassium channel 1
NCBI Official Symbol
HCN1
NCBI Official Synonym Symbols
BCNG1; HAC-2; BCNG-1; EIEE24; GEFSP10
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Gene Name
HCN1
UniProt Synonym Gene Names
BCNG1; BCNG-1
UniProt Entry Name
HCN1_HUMAN
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Product Notes
The HCN1 hcn1 (Catalog #AAA328244) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-HCN1 (Ser846) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's HCN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HCN1 hcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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