WB (Western Blot)
(Anti- HNF1 antibody, AAA46045, Western blottingAll lanes: Anti HNF1 (AAA46045) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: RH35 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 67KDObserved bind size: 67KD)
Rabbit Hepatocyte nuclear factor 1-alpha Polyclonal Antibody | anti-HNF1A antibody
Anti-HNF1 Antibody
Gene Names
HNF1A; HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Hepatocyte nuclear factor 1-alpha, Antibody; Anti-HNF1 Antibody; Albumin proximal factor antibody; Hepatic nuclear factor 1 alpha antibody; Hepatic nuclear factor 1 antibody; Hepatic transcription factor 1 alpha antibody; Hepatic transcription factor 1 antibody; Hepatocyte nuclear factor 1-alpha antibody; HNF 1 antibody; HNF 1A antibody; HNF-1-alpha antibody; HNF-1A antibody; HNF1A antibody; HNF1A_HUMAN antibody; Interferon production regulator factor antibody; LF B1 antibody; LF B1 hepatic nuclear factor antibody; LFB 1 antibody; LFB1 antibody; LFB1 hepatic nuclear factor antibody; Liver specific transcription factor LF B1 antibody; Liver specific transcription factor LFB1 antibody; Liver-specific transcription factor LF-B1 antibody; Maturity onset diabetes of the young 3 antibody; MODY 3 antibody; MODY3 antibody; TCF 1 antibody; TCF-1 antibody; TCF1 antibody; Transcription factor 1 antibody; Transcription factor 1 hepatic antibody; HNF1 homeobox A; anti-HNF1A antibody
Host
Rabbit
Reactivity
Human, Rat. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
631
Applicable Applications for anti-HNF1A antibody
WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human HNF1 recombinant protein (Position: A431-Q631). Human HNF1 shares 93% and 92% amino acid (aa) sequences identity with mouse and rat HNF1, respectively.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti- HNF1 antibody, AAA46045, Western blottingAll lanes: Anti HNF1 (AAA46045) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: RH35 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 67KDObserved bind size: 67KD)
WB (Western Blot)
(Anti- HNF1 antibody, AAA46045, Western blottingAll lanes: Anti HNF1 (AAA46045) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: RH35 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 67KDObserved bind size: 67KD)
WB (Western Blot)
(Anti- HNF1 antibody, AAA46045, Western blottingAll lanes: Anti HNF1 (AAA46045) at 0.5ug/mlWB: Recombinant Human HNF1 Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD)
WB (Western Blot)
(Anti- HNF1 antibody, AAA46045, Western blottingAll lanes: Anti HNF1 (AAA46045) at 0.5ug/mlWB: Recombinant Human HNF1 Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD)
Related Product Information for anti-HNF1A antibody
Description: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 1-alpha(HNF1A) detection. Tested with WB in Human;Rat.
Background: HNF1 homeobox A (hepatocyte nuclear factor 1 homeobox A), also known as HNF1A or TCF1, is a human gene. It is mapped to chromosome 12q24.3. The protein encoded by this gene is a transcription factor that is highly expressed in the liver and is involved in the regulation of the expression of several liver-specific genes. HNF1A can function as a dimer. What's more, deletion of the HNF1A activation domains or interruption of the HNF1-binding sites in the LCT promoter resulted in complete loss of transcriptional activity.
Background: HNF1 homeobox A (hepatocyte nuclear factor 1 homeobox A), also known as HNF1A or TCF1, is a human gene. It is mapped to chromosome 12q24.3. The protein encoded by this gene is a transcription factor that is highly expressed in the liver and is involved in the regulation of the expression of several liver-specific genes. HNF1A can function as a dimer. What's more, deletion of the HNF1A activation domains or interruption of the HNF1-binding sites in the LCT promoter resulted in complete loss of transcriptional activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,491 Da
NCBI Official Full Name
hepatocyte nuclear factor 1-alpha
NCBI Official Synonym Full Names
HNF1 homeobox A
NCBI Official Symbol
HNF1A
NCBI Official Synonym Symbols
HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
NCBI Protein Information
hepatocyte nuclear factor 1-alpha; HNF-1-alpha; albumin proximal factor; hepatic nuclear factor 1; transcription factor 1, hepatic; interferon production regulator factor; liver-specific transcription factor LF-B1
UniProt Protein Name
Hepatocyte nuclear factor 1-alpha
UniProt Gene Name
HNF1A
UniProt Synonym Gene Names
TCF1; HNF-1-alpha; HNF-1A; LFB1; TCF-1
UniProt Entry Name
HNF1A_HUMAN
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Product Notes
The HNF1A hnf1a (Catalog #AAA46045) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HNF1 Antibody reacts with Human, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatocyte nuclear factor 1-alpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the HNF1A hnf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatocyte nuclear factor 1-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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