IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HNF1A Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit HNF1A Polyclonal Antibody | anti-HNF1A antibody
HNF1A Antibody (Center)
Gene Names
HNF1A; HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HNF1A, Antibody; HNF1A Antibody (Center); Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; Liver-specific transcription factor LF-B1; LFB1; Transcription factor 1; TCF-1; HNF1A; TCF1; anti-HNF1A antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HNF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the Central region of human HNF1A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
177-205
Sequence Length
119
Applicable Applications for anti-HNF1A antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HNF1A Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HNF1A Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of HNF1A Antibody (Center) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of HNF1A Antibody (Center) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab.)
Related Product Information for anti-HNF1A antibody
HNF1A is a transcription factor required for the expression of several liver-specific genes. This protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
References
Bach,I., et.al., Genomics 8 (1), 155-164 (1990)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67356
NCBI Official Full Name
hepatocyte nuclear factor 1-alpha isoform 2
NCBI Official Synonym Full Names
HNF1 homeobox A
NCBI Official Symbol
HNF1A
NCBI Official Synonym Symbols
HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
NCBI Protein Information
hepatocyte nuclear factor 1-alpha
UniProt Protein Name
Hepatocyte nuclear factor 1-alpha
UniProt Gene Name
HNF1A
UniProt Synonym Gene Names
TCF1; HNF-1-alpha; HNF-1A; LFB1; TCF-1
UniProt Entry Name
HNF1A_HUMAN
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Product Notes
The HNF1A hnf1a (Catalog #AAA290233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 177-205. The HNF1A Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's HNF1A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HNF1A hnf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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