WB (Western Blot)
(Detection of human HERC2 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse HERC2 Polyclonal Antibody | anti-HERC2 antibody
Rabbit anti-HERC2 Antibody, Affinity Purified
Gene Names
HERC2; jdf2; p528; MRT38; SHEP1; D15F37S1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HERC2, Antibody; Rabbit anti-HERC2 Antibody, Affinity Purified; hect domain and RCC1-like domain-containing protein 2; SHEP1; probable E3 ubiquitin-protein ligase HERC2; p528; MRT38; hect domain and RLD 2; E3 ubiquitin-protein ligase HERC2; D15F37S1; jdf2; HECT-type E3 ubiquitin transferase HERC2; HECT and RLD domain containing E3 ubiquitin protein ligase 2; anti-HERC2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-HERC2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 4784 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HERC2 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human HERC2 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse HERC2 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse HERC2 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human HERC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for IP at 6 ug per reaction. HERC2 was also immunoprecipitated by a previous lot of this antibody (AAA211674 lot 2) and a second antibody against a different epitope of HERC2 (BL7565). For blotting immunoprecipitated HERC2, AAA211674 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human HERC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC2 antibody (AAA211674 lot 3) used for IP at 6 ug per reaction. HERC2 was also immunoprecipitated by a previous lot of this antibody (AAA211674 lot 2) and a second antibody against a different epitope of HERC2 (BL7565). For blotting immunoprecipitated HERC2, AAA211674 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-HERC2 antibody
HERC2 (HECT domain and RCC1-like domain-containing protein 2) belongs to the HERC family of ubiquitin ligases. A single nucleotide polymorphism (SNP) in an evolutionarily conserved region of the HERC2 gene is associated with blue eye color. Additionally, it has been reported that gene-gene interactions between HERC2, OCA2, and MC1R may influence eye, skin, and hair color.
Product Categories/Family for anti-HERC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
527,228 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase HERC2
NCBI Official Synonym Full Names
HECT and RLD domain containing E3 ubiquitin protein ligase 2
NCBI Official Symbol
HERC2
NCBI Official Synonym Symbols
jdf2; p528; MRT38; SHEP1; D15F37S1
NCBI Protein Information
E3 ubiquitin-protein ligase HERC2
UniProt Protein Name
E3 ubiquitin-protein ligase HERC2
UniProt Gene Name
HERC2
UniProt Entry Name
HERC2_HUMAN
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Product Notes
The HERC2 herc2 (Catalog #AAA211674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HERC2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HERC2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HERC2 herc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HERC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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