WB (Western Blot)
(The cell lysate derived from HELA was immunoprobedbyeither Rabbit anti-beta-Catenin (pS675) (Lane 1) or theantibody pre-incubatedby immunization peptide (lane 2) at 1:500 dilution.)
Rabbit Catenin-b Polyclonal Antibody
Rabbit anti Catenin-b (pS675) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Purified by site-modified Epitope Affinity Purification.
Synonyms
Catenin-b, Antibody; Rabbit anti Catenin-b (pS675) Polyclonal Antibody; CTNNB1; anti-Catenin-b antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by site-modified Epitope Affinity Purification.
Form/Format
Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-Catenin-b antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Preparation
A synthetic peptide corresponding to the epitope -KRLSVEL- with a single phosphorylation site Ser675 of human b-catenin.
Specificity
This antibody recognizes ~88 kDa of phosphorylated human beta-catenin protein at Ser 675. It also reacts with mouse rat. The other species are not tested.
Positive Control
HELA cell lysate
Cellular Location
Cell membrane/cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C to 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
WB (Western Blot)
(The cell lysate derived from HELA was immunoprobedbyeither Rabbit anti-beta-Catenin (pS675) (Lane 1) or theantibody pre-incubatedby immunization peptide (lane 2) at 1:500 dilution.)
WB (Western Blot)
(The cell lysate derived from HELA was immunoprobedbyeither Rabbit anti-beta-Catenin (pS675) (Lane 1) or theantibody pre-incubatedby immunization peptide (lane 2) at 1:500 dilution.)
Related Product Information for anti-Catenin-b antibody
Beta-catenin, a cytosolic, 88 kDa, 781 amino acid protein, belongs to the beta-catenin family. The N-terminus domain, contains the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin (s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280, S675, etc. Phosphorylation of beta-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
References
Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.
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Product Notes
The Catenin-b (Catalog #AAA71321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Catenin-b (pS675) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Catenin-b can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the Catenin-b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catenin-b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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