IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using HFE antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human, Mouse HFE Polyclonal Antibody | anti-HFE antibody
HFE Polyclonal Antibody
Gene Names
HFE; HH; HFE1; HLA-H; MVCD7; TFQTL2
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
HFE, Antibody; HFE Polyclonal Antibody; HH; HFE1; HLA-H; MVCD7; TFQTL2; MGC103790; dJ221C16.10.1; anti-HFE antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
348
Applicable Applications for anti-HFE antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-306 of human HPE (NP_000401.1)
Category
Polyclonal Antibody
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Preparation and Storage
Store at -20 degree. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using HFE antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using HFE antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HFE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HFE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-HFE antibody
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-HFE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 50 kDa
Calculated MW: 40kDa
Calculated MW: 40kDa
NCBI Official Full Name
Hereditary hemochromatosis protein
NCBI Official Synonym Full Names
hemochromatosis
NCBI Official Symbol
HFE
NCBI Official Synonym Symbols
HH; HFE1; HLA-H; MVCD7; TFQTL2
NCBI Protein Information
hereditary hemochromatosis protein; high Fe; MHC class I-like protein HFE; hereditary hemochromatosis protein HLA-H
UniProt Protein Name
Hereditary hemochromatosis protein
UniProt Gene Name
HFE
UniProt Synonym Gene Names
HLAH
UniProt Entry Name
HFE_HUMAN
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Product Notes
The HFE hfe (Catalog #AAA37157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HFE Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HFE can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HFE hfe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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