Rabbit HHV11 ICP22 Polyclonal Antibody | anti-HHV11 antibody

HHV11 ICP22 Polyclonal Antibody

Cat. Num. AAA291623

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HHV11 ICP22, Antibody; HHV11 ICP22 Polyclonal Antibody; Transcriptional regulator ICP22; Immediate-early protein IE68; Infected cell protein 22; ICP22; anti-HHV11 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glycerol

• Applicable Applications for anti-HHV11 antibody: ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Function: Transcriptional regulator that interacts with cellular elongation regulators to inhibit host cell transcription and promote viral gene expression in productive infection, mainly by mediating changes on the host RNA polymerase II (PubMed:17344289, PubMed:28611249, PubMed:34696162). One change, which is UL13 independent, is the rapid loss of Pol II forms bearing Ser-2 phosphorylation in the heptapeptides of the CTD leading to unproductive elongation of pol II (PubMed:17344289, PubMed:25233083). A second change, which is UL13 dependent, is the appearance of an intermediate form of Pol II that differs from the normal hypo- and hyperphosphorylated forms. These Pol II modifications immediately inhibit host genome transcription, leading to cell cycle deregulation and loss of efficient antiviral response (Probable). In opposition to its role in productive infection, inhibits transcription elongation of early viral genes, which can be beneficial because it reduces antisense and intergenic transcription (PubMed: , PubMed: ). This regulatory function directly or indirectly helps to retain Pol activity on the viral genome later in infection (PubMed:35019725). The viral protein VP16 overcomes transcriptional inhibition by ICP22 to promote transcription of IE genes (PubMed: ,PubMed: ). Recruits FACT complexes to the viral genome by interacting with the FACT complex, which could allow Pol II to cross the nucleosome barrier on the viral genome and achieve efficient viral transcription elongation late in viral infection and ultimately infectious virion production (Probable). Plays a role in virion primary envelopment, possibly by interacting with and regulating UL31 and UL34 (PubMed: ). Essential for the recruitment of Hsc70 into virus induced chaperone enriched (VICE) domains in infected cells (PubMed:20032172). May function as a co-chaperone (J protein-like), functionning together with Hsc70 to recognize and manage aggregated and misfolded proteins (PubMed: ).
• Cellular Location: Host nucleus Note=Localizes in small nuclear bodies early in infection then moves to a more diffuse distribution in viral compartments as infection progresses (PubMed:16877770). UL31 mediates the recruitment and anchorage of ICP22 at the nuclear membrane (PubMed:24741100)
• Preparation and Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.

NCBI and Uniprot Product Information

• NCBI GI #: 820945291
• NCBI GeneID: 2703435
• NCBI Accession #: YP_009137136.1
• NCBI GenBank Nucleotide #: NC_001806.2
• UniProt Accession #: P04485
• NCBI Official Full Name: regulatory protein ICP22
• NCBI Official Symbol: US1
• NCBI Protein Information: required for expression of a subset of late genes; involved in gene regulation; involved in cell cycle regulation
• UniProt Protein Name: Transcriptional regulator ICP22
• UniProt Gene Name: ICP22
• UniProt Synonym Gene Names: ICP22