FCM/FACS (Flow Cytometry)
(Dilution: Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-HIF3 alpha antibody (bs-5898R),ilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1 ug /test.)
Rabbit HIF3 alpha Polyclonal Antibody | anti-HIF3alpha antibody
HIF3 alpha Polyclonal Antibody
Gene Names
HIF3A; IPAS; MOP7; PASD7; HIF-3A; bHLHe17
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HIF3 alpha, Antibody; HIF3 alpha Polyclonal Antibody; Hypoxia-inducible factor 3-alpha; HIF-3-alpha; HIF3-alpha; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7; HIF3A; BHLHE17; MOP7; PASD7; anti-HIF3alpha antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-HIF3alpha antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Dilution: Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-HIF3 alpha antibody (bs-5898R),ilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1 ug /test.)
FCM/FACS (Flow Cytometry)
(Dilution: Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-HIF3 alpha antibody (bs-5898R),ilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1 ug /test.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated (bs-5898R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated (bs-5898R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Sample:Lung (Mouse) Lysate at 40 ugPrimary: Anti-HIF3 alpha (bs-5898R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 74 kDObserved band size: 75 kD)
WB (Western Blot)
(Sample:Lung (Mouse) Lysate at 40 ugPrimary: Anti-HIF3 alpha (bs-5898R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 74 kDObserved band size: 75 kD)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,433 Da
NCBI Official Full Name
hypoxia-inducible factor 3-alpha isoform c
NCBI Official Synonym Full Names
hypoxia inducible factor 3, alpha subunit
NCBI Official Symbol
HIF3A
NCBI Official Synonym Symbols
IPAS; MOP7; PASD7; HIF-3A; bHLHe17
NCBI Protein Information
hypoxia-inducible factor 3-alpha; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17
UniProt Protein Name
Hypoxia-inducible factor 3-alpha
UniProt Gene Name
HIF3A
UniProt Synonym Gene Names
BHLHE17; MOP7; PASD7; HIF-3-alpha; HIF3-alpha; bHLHe17; IPAS
UniProt Entry Name
HIF3A_HUMAN
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Product Notes
The HIF3alpha hif3a (Catalog #AAA292017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF3 alpha Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HIF3 alpha can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HIF3alpha hif3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF3 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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