WB (Western Blot)
(Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37? C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Rabbit anti-Human, Mouse HIF3A / HIF3-Alpha Polyclonal Antibody | anti-HIF3A antibody
Rabbit Polyclonal to Mouse HIF3A / HIF3-Alpha
Gene Names
HIF3A; IPAS; MOP7; PASD7; HIF-3A; bHLHe17
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
HIF3A / HIF3-Alpha, Antibody; Rabbit Polyclonal to Mouse HIF3A / HIF3-Alpha; Anti-HIF3A / HIF3-Alpha Antibody (aa581-592) IHC-plus; HIF3A; BHLHe17; HIF-3A; IPAS; HIF3-alpha-1; Inhibitory PAS domain protein; PASD7; HIF-3-alpha; HIF3-alpha; Member of PAS protein 7; MOP7; Mouse HIF3A; HIF3-Alpha; anti-HIF3A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
669
Applicable Applications for anti-HIF3A antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Mouse
Immunogen Description
Synthetic peptide from mouse HIF3A.
Immunogen Type
Synthetic peptide
Immunogen
HIF3A / HIF3-Alpha antibody was raised against synthetic peptide from mouse HIF3A.
Antigen Modification
aa581-592
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37? C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37? C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
IHC (Immunohiostchemistry)
(Anti-HIF3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohiostchemistry)
(Anti-HIF3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HIF3A antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HIF3A antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,359 Da
NCBI Official Full Name
hypoxia-inducible factor 3-alpha isoform c
NCBI Official Synonym Full Names
hypoxia inducible factor 3, alpha subunit
NCBI Official Symbol
HIF3A
NCBI Official Synonym Symbols
IPAS; MOP7; PASD7; HIF-3A; bHLHe17
NCBI Protein Information
hypoxia-inducible factor 3-alpha; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17
UniProt Protein Name
Hypoxia-inducible factor 3-alpha
UniProt Gene Name
HIF3A
UniProt Synonym Gene Names
BHLHE17; MOP7; PASD7; HIF-3-alpha; HIF3-alpha; bHLHe17; IPAS
UniProt Entry Name
HIF3A_HUMAN
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Product Notes
The HIF3A hif3a (Catalog #AAA50777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse HIF3A / HIF3-Alpha reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HIF3A / HIF3-Alpha can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HIF3A hif3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF3A / HIF3-Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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